LOCUS       sequence-76559-e         666 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Cumate-inducible expression of mCherry-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQR
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    148..175
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    218..245
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
ORIGIN
        1 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
       61 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
      121 caccatgcgg aagcttcctc tactagttac aaacagacca gattgtctgt ttgttaaccg
      181 gcgcagttag cggcttcgcg gcgatccgtc aaccatgtac aaacagacca gattgtctgt
      241 ttgttccggt acattgacat atgtatccgc tcatgataca ataaccctga tgctagcagg
      301 gagagacccc gaatggtcat catgtcgccg aagcgccgca cccaggccga gcgcgcgatg
      361 gaaacccagg gcaagctgat cgccgccgcc ctgggcgtcc tgcgcgagaa gggctatgcc
      421 ggcttccgca tcgccgacgt cccgggcgcc gccggcgtca gccgcggcgc ccagtcgcac
      481 cacttcccga ccaagctgga actgctgctg gccaccttcg agtggctgta cgagcagatc
      541 accgagcgca gccgcgcccg cctggccaag ctgaagcccg aggacgacgt catccagcag
      601 atgctggacg acgccgccga gttcttcctg gacgacgact tctcgatctc gctggacctg
      661 atcgtg
//
