LOCUS       sequence-76609-e         835 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH72400
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     206..223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      319..519
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     primer_bind     complement(559..577)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        645..749
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      241 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct gtggtctcag
      301 cttgatcccc cgtcgacagc tagctatatc atcaatttat gtattacaca taatatcgca
      361 ctcagtcttt catctacggc aatgtaccag ctgatataat cagttattga aatatttctg
      421 aatttaaact tgcatcaata aatttatgtt tttgcttgga ctataatacc tgacttgtta
      481 ttttatcaat aaatatttaa actatatttc tttcaagatc gcttgagacc acgaagtggc
      541 tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      601 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      661 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      721 tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga
      781 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactg
//
