LOCUS       sequence-76664-e         926 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL13001
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(41..386)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(78..98)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tttcgtccac tgaagagcca cttcgtggtc tcaatgggcg tgtcctctcc aaatgaaatg
       61 aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta
      121 cgtcagtgga gatgtcacat caatccactt gctttgtaga cgtggttgga acctcttctt
      181 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagagagat
      241 cttgaatgat agcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt
      301 ctactgtcct ttcgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
      361 aaattatcct ttgttgaaaa gtctcaatag ccctttgatc ttctgagact gtatctttga
      421 catttttgga gtagaccaga gtgtcgtgct ccaccatgtt gacgaagatt ttcttcttgt
      481 cattgagtcg taaaagactc tgtatgaact gttcgccagt cttgacggcg agttctgtta
      541 gatcctcgat ttgaatctta gactccatgc atggccttag attcagtagg aactaccttt
      601 ttagagactc caatctctat tacttgcctt ggtttatgaa gcaagccttg aatcgtccat
      661 actggaatag tacttctgat cttgagaaat atgtctttct ctgtgttctt gatgcaatta
      721 gtcctgaatc ttttgactgc atctttaacc ttcttgggaa ggtatttgat ctcctggaga
      781 ttgttactcg ggtagatcgt cttgatgaga cttgctgcgt aggcctctct aaccatctgt
      841 gggtcagcat tctttctgaa attgaagagg ctaaccttct cattatcagt ggtgaacata
      901 gtgtcgtcac cttcaccttc gaactt
//
