LOCUS       sequence-76993-e        1076 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  this report is the first to directly measure nAChr subunit 
            stoichiometry using FRET and plasma membrane localization of Alpha6 
            and Beta3 containing receptors using TIRF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Drenan RM, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester HA
  TITLE     Subcellular trafficking, pentameric assembly, and subunit 
            stoichiometry of neuronal nicotinic acetylcholine receptors 
            containing fluorescently labeled alpha6 and beta3 subunits.
  JOURNAL   Mol Pharmacol. 2008 Jan;73(1):27-41. Epub 2007 Oct 11.
  PUBMED    17932221
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2008-01"; volume: "73(1)"; pages: "27-41. Epub 
            2007 Oct 11"
COMMENT     Sequence Label: pcDNA3.1-nAChr-Alpha6-CFP
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(300..1010,1011..1013,1014..1016))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(342..363)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     691..710
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     950..971
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acgggccctc tagactcgag ttaagacttt cctgtgttcc caagcagtgg ctgcagaaat
       61 agccccacag ttccaaacac acagacaatt ataaataccc aaaggaagac tctgtccacc
      121 accatagcca tgtatttcca gtcgtcttct acctcgtttg tttcattgtg gctcttcatg
      181 ttttctgcta tgaattgaac actctcgatg acatcttcaa catcggacga gtgctctgaa
      241 ttctctgcca cccaccgtgc aggctgctgg cttgatcttc tctttccagg tgctcctgcc
      301 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      361 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      421 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      481 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttggcctt gatgccgttc
      541 ttctgcttgt cggcggtgat atagacgttg tggctgatgt agttgtactc cagcttgtgc
      601 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      661 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      721 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      781 atgtggtcgg ggtagcggct gaagcactgc acgccccagg tcagggtggt cacgagggtg
      841 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
      901 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      961 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccattgct
     1021 cctgctgcta tgtcacttga tttttggcag tggtggcatt cctttagaag tttgga
//
