LOCUS       sequence-77036-e         794 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Gong Y, Wagner MJ, Zhong Li J, Schnitzer MJ
  TITLE     Imaging neural spiking in brain tissue using FRET-opsin protein 
            voltage sensors.
  JOURNAL   Nat Commun. 2014 Apr 22;5:3674. doi: 10.1038/ncomms4674.
  PUBMED    24755708
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-04-22"; volume: "5:3674. doi"; pages: " 
            10.1038/ncomms4674"
COMMENT     Sequence Label: pLenti-CamkII-MacQ-mCitrine
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(135..386)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(177..198)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     526..545
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattctca ttacacctcg
       61 ttctcgtagc agaacacgtt gatgtcgatc tggtccaggg ggatgtactc gccctcgctg
      121 gtgatcctgc tcttcttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      181 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag tttggactgg
      241 tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      301 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      361 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
      421 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      481 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      541 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
      601 aagtcgtgct gcttcatgtg gtcggggtag cgcgcgaagc acatcaggcc gtagccgaag
      661 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
      721 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
      781 tttacgtcgc cgtc
//
