LOCUS       sequence-77668-e        7135 bp    DNA     circular SYN 14-APR-2025
DEFINITION  bi-directional expression vector for yeast, galactose inducible, 
            ADE2 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Miller CA 3rd, Martinat MA, Hyman LE
  TITLE     Assessment of aryl hydrocarbon receptor complex interactions using 
            pBEVY plasmids: expressionvectors with bi-directional promoters for 
            use in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.
  PUBMED    9671822
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998-08-1"; volume: "26(15)"; pages: "3577-83"
COMMENT     Sequence Label: pBEVY-GA
FEATURES             Location/Qualifiers
     source          1..7135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(267..454)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     375..399
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        624..1288
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    840..957
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     1252..1275
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      1325..1624
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     rep_origin      1649..2813
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3095..4810)
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     promoter        complement(4811..5186)
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     promoter        5229..5333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5334..5402,5403..6194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5552..5571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6365..6953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6854..6873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7107..7124
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctttt
      241 catagggtag gggaatttcg ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat
      301 gctatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaac aagaattttc
      361 gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt
      421 taataataaa aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc
      481 taccaacgat ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga
      541 caaccttgat tggagacttg accaaacctc tggcgaagaa gtccaaagct tgcatgcctg
      601 caggtcgact ctagaggatc ccctttcaaa aattcttact ttttttttgg atggacgcaa
      661 agaagtttaa taatcatatt acatggcatt accaccatat acatatccat atacatatcc
      721 atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa
      781 aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac
      841 ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc
      901 tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac
      961 aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct
     1021 ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt
     1081 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta
     1141 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg
     1201 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct
     1261 ctatacttta acgtcaagga gaaaaaaccc gggtaccgag ctcgaattcg acacttctaa
     1321 ataagcggat ctcctatgcc ttcacgattt atagtttcca ttatcaagta tgcctatatt
     1381 agtatatagc atctttagat gacagtgttc gaagtttcac gaataaaaga taatattcta
     1441 ctttttgctc ccaccgcgtt tgctagcacg agtgaacacc atccctcgcc tgtgagttgt
     1501 acccattcct ctaaactgta gacatggtag cttcagcagt gttcgttatg tacggcatcc
     1561 tccaacaaac agtcggttat agtttgtcct gctcctctga atcgtctccc tcgatatttc
     1621 tcattttcct tcgcatgccc tcgagcggaa cgaagcatct gtgcttcatt ttgtagaaca
     1681 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     1741 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     1801 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     1861 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     1921 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     1981 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     2041 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     2101 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     2161 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     2221 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     2281 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
     2341 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
     2401 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
     2461 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
     2521 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
     2581 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gctagagaat
     2641 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
     2701 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
     2761 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata
     2821 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
     2881 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
     2941 ccgatcttat gtatgaaatt cttaaaaaag gacacctgta agcgttgatt tctatgtatg
     3001 aagtccacat ttgatgtaat cataacaaag cctaaaaaat aggtatatca ttttataatt
     3061 atttgctgta caagtatatc aataaactta tatattactt gttttctaga taagcttcgt
     3121 aaccgacagt ttctaacttt tgtgctttga caagaacttc ttcttcttgc tttaataaaa
     3181 actgttccat tttcgttgta taacttgaat cataagcgcc aagcagtctg acagccaaca
     3241 gcgcagcgtt cgtactatta ttaatagcga cggtagctac tggaacacct ctaggcattt
     3301 gcacaattga atgtaaagaa tctactccat ctagacaaga accttttacg ggcacaccga
     3361 tgacaggaag tggtgtcatt gcagccacca tacctggcaa gtgagcagcc ccaccagctc
     3421 cagcgataat tgttttaatt ccacgcttgc ttgcggaaat agcatatgct gacatcctat
     3481 gtggagttct atgagcagag actattgtca cttcaaatgg aacgccaaaa tcttttaaaa
     3541 ccgcacatgc ggcagacatt accggcaagt cagagtctga tcccatgatg attccaacca
     3601 atggtttgac cattgcttcc aagtccaact tttgagcgac agagattttg attggaatat
     3661 cagttctacc tgtaatgtag ttcagccttt gttcacattc cgccatactg gaggcaataa
     3721 tatttatgtg acctactttt ctgttaggtc tagactcttt tccatataag tacactgagg
     3781 aacctggagt cgccaatgct ctttcgcaag tttctagctc tttatctttt gtatgtttgt
     3841 ctccaagaac atttagcata atggcgttcg ttgtaatggt ggagaaagat gtgaaattct
     3901 ttggcattgg caaatccaat attgatctca aatgagcttc aaattgagaa gtgacgcaag
     3961 catcaatggt ataatgtcca gagttgtgag gccttggggc aatttcgtta ataagcaatt
     4021 cccctgtttc taaatagaac atttccacac caaatatacc acaaccggga aaagatttga
     4081 ttgcattttc tgccaacaac ttcgccttaa gttgaacgga gtccggaact ctagcaggcg
     4141 cataacataa gtcacaaata ttgtccttgt ggatagtctc tacaattggg taagaaaaca
     4201 ctaaaccgtt aacagacctc acaatcatga ctgctaattc tttagtaaat ggtgcccatt
     4261 tttcggcgta caaaggacga tccttcagta cttccaaagc ttccggaatc atttccttat
     4321 tctttacaac gaagttacct cttccatcgt atgccaaagt cctcgacttc aagacgaatg
     4381 gaaaacccaa atctcttcca acattcaata gggacgtctc actggcttgt tccacaggaa
     4441 cactttgggt aactgctata ccatttttga ttaaatgctc tttttgaata tatttgtctt
     4501 gtatcaatct gattgtttct ggagaagggt aaatttttaa tttgggatgt tttacttgaa
     4561 gattctttag tgtaggaaca tcaacatgct caatctcaat cgttagcaca tcacattttt
     4621 cagctagttt ttcgatatca agaggattgg aaaaggagcc attaacgtgg tcattggagt
     4681 tgcttatttg tttggcagga gaattttcag catctagtat taccgtctta atgttgagcc
     4741 tgtttgctgc ctcaacaatc atacgtccca attgtccccc tcctaatata ccaactgttc
     4801 tagaatccat acttgattgt tttgtccgat tttcttgttt ttcttgattg ttatagtagg
     4861 atgtacttag aagagagatc caacgatttt acgcaccaat ttatacatga aatgctccat
     4921 aatattgtcc atttagttct taataaaagg tcagcaagag tcaatcactt agtattaccc
     4981 ggttcgtagc catgcaacaa gagtcatttg tcagcatagc tgtaataatc aatcatgacg
     5041 taagaaatgt atcataatta aaagttgtta aagatgtcag tgttatgttg gtgttacaaa
     5101 attctcggct tctcactaat atttaatatc tcttaaattt tatctgtctt tgattctttt
     5161 aagaaaagtt atgtattatt caagaaaaag tcaattcaga tccaggtggc acttttcggg
     5221 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5281 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5341 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5401 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5461 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5521 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5581 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5641 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5701 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5761 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5821 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5881 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5941 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6001 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6061 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6121 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6181 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6241 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6301 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6361 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6421 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6481 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     6541 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6601 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6661 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6721 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6781 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6841 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6901 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6961 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7021 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7081 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//
