LOCUS       sequence-77960-e         599 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Modified pBluescript containing a telomeric insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Arat NO, Griffith JD
  TITLE     Human Rap1 interacts directly with telomeric DNA and regulates TRF2 
            localization at the telomere.
  JOURNAL   J Biol Chem. 2012 Dec 7;287(50):41583-94. doi: 
            10.1074/jbc.M112.415984. Epub 2012 Oct 20.
  PUBMED    23086976
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.415984"; journalName: "J Biol Chem"; date: 
            "2012-12-7- 7"; volume: "287"; issue: "50"; pages: "41583-94"
COMMENT     Sequence Label: f.pRST1
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(174..190)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(174..190)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(227..247)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(227..245)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(266..282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(266..282)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(279..301)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    290..306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(314..320,321..338,339..344))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    359..380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(497..514)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gacggtatcg ataagcttga agacttaggg ttagggttag ggttagggtt agggttaggg
       61 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
      121 ttagggttag ggttagggtt agggttaggg ttagggttag ggagacgaat tcggatccac
      181 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
      241 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      301 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      361 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      421 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      481 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      541 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgca
//
