LOCUS       sequence-78387-e        5923 bp    DNA     circular SYN 14-APR-2025
DEFINITION  retroviral expression of mouse Sox17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, 
            Okita K, Mochiduki Y, Takizawa N, Yamanaka S
  TITLE     Generation of induced pluripotent stem cells without Myc from mouse 
            and human fibroblasts.
  JOURNAL   Nat Biotechnol. 2008 Jan;26(1):101-6. Epub 2007 Nov 30.
  PUBMED    18059259
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "2008-01"; volume: "26(1)"; pages: "101-6. Epub 
            2007 Nov 30"
COMMENT     Sequence Label: pMXs-ms-Sox17
FEATURES             Location/Qualifiers
     source          1..5923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1100..1516
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1446..1468
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1484..1500
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1526..1900
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1838..1854
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1951..1975
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3272..3296)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             3368..3961
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4027..4044)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4198..4786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4278..4297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4950..5741,5742..5810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5573..5592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5811..5915)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggatctcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca
     1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1501 tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct
     1561 tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca
     1621 cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca
     1681 agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt
     1741 ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct
     1801 gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt
     1861 gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt
     1921 aaggatccca gtgtggtggt acgggaaatc acaagtttgt acaaaaaagc aggctccgcg
     1981 gccgccccct tcaccatgag cagcccggat gcgggatacg ccagtgacga ccagagccag
     2041 ccccggagcg cgcagcccgc ggtgatggca gggttgggcc cctgtccctg ggccgagtcc
     2101 ctgagccccc tcggggatgt aaaggtgaaa ggcgaggtgg tggcgagtag cggggcgcca
     2161 gccgggacgt cgggccgagc caaagcggag tctcgcatcc ggcggccgat gaacgccttt
     2221 atggtgtggg ccaaagacga acgcaagcgg ttggcacagc agaacccaga tctgcacaac
     2281 gcagagctaa gcaagatgct aggcaagtct tggaaggcgt tgaccttggc agagaagcgg
     2341 cccttcgtgg aagaggccga gcggctgcgc gtgcagcata tgcaggacca ccccaactac
     2401 aagtaccggc cgcggcggcg caagcaggtg aagcgcatga agcgggtgga gggaggcttc
     2461 ctgcacgctc tcgtcgagcc ccaggccggc gcgcttggtc ccgagggcgg ccgcgtggcc
     2521 atggatggcc tgggtctgcc tttcccggag ccgggctatc cggccggtcc tccgctgatg
     2581 tctccgcaca tgggccccca ctatcgggac tgccagggac tgggcgctcc cgcgctcgac
     2641 ggctaccctc tgcccactcc ggacacatcc ccgctggatg gcgtggagca ggacccggct
     2701 ttctttgcag ccccgctgcc aggggactgc ccggcggccg gcacctacac ttacgctcca
     2761 gtctcggact atgcagtgtc cgtagagccg cccgctggcc ccatgcgagt ggggccggac
     2821 ccctcgggcc ctgcgatgcc ggggatcctg gcgcccccca gcgctctgca cctgtactac
     2881 ggcgcgatgg gctcgcccgc cgcaagtgcg gggcgcggtt tccacgcgca accccagcag
     2941 ccgctgcaac cgcaggcacc gccgccgcca ccgcagcagc agcacccagc gcacggcccc
     3001 gggcaacctt cgccccctcc cgaggctctg ccctgccggg atggcacgga atccaaccag
     3061 cccactgagc tcctagggga ggtggaccgc acggaattcg aacagtatct gccctttgtg
     3121 tataagcccg agatgggtct tccctaccag ggacacgact gcggagtgaa cctctcagac
     3181 agccacggag ccatttcctc cgtggtgtcc gacgctagct cagcggtcta ctattgcaac
     3241 taccccgaca tttgaaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggtgatg
     3301 agcggccgcc agcacagtgg tcgacgataa aataaaagat tttatttagt ctccagaaaa
     3361 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     3421 ggaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     3481 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     3541 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     3601 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     3661 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     3721 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     3781 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     3841 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     3901 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
     3961 tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4021 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4081 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4141 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4201 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4261 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4321 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4381 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4441 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4501 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4561 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4621 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4681 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4741 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4801 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4861 gagattatca aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa
     4921 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4981 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5041 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5101 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5161 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5221 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5281 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5341 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5401 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5461 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5521 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     5581 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5641 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5701 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5761 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5821 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5881 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttc
//
