LOCUS       sequence-78594-e         873 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  ePathBrick vector for pathway engineering in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Xu P, Vansiri A, Bhan N, Koffas MA
  TITLE     ePathBrick: a synthetic biology platform for engineering metabolic 
            pathways in E. coli.
  JOURNAL   ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. 
            Epub 2012 May 4.
  PUBMED    23651248
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300016b";
            journalName: "ACS Synth Biol"; date: "2012-07-20- 20"; volume: "1"; 
            issue: "7"; pages: "256-66"
COMMENT     Sequence Label: pETM6
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             211..255
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(294..312)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      308..355
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      399..854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     696..717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggccacgatg cgtccggcgt agcctaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat acatatggca gatctcaatt ggatatcggc cggccacgcg
      181 atcgctgacg tcggtaccct cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc
      241 cagcacatgg actcgtctac tagtcgcagc ttaattaacc taaactgctg ccaccgctga
      301 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctagc
      361 gaaaggagga gtcgactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      421 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      481 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      541 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      601 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      661 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      721 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
      781 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
      841 aacgtttaca atttctggcg gcacgatggc atg
//
