LOCUS       sequence-78618-e       12414 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Single component LSL vector with GFP. Rep is expressed from the 
            complementary sense LIR promoter. GFP is between LIR and SIR and is 
            driven from a 2X35S promoter. Rep is within the replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 12414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLGFP.R
FEATURES             Location/Qualifiers
     source          1..12414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1129..1323
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1559..1581)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1667..1807
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1722..1741
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1822..1839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1993..2581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2073..2092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2668..3462)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3887..3911
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    3989..4163
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(5253..5930)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        6946..7291
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7339..7367)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7962..7982
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      8041..8293
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10023..10047
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11347..11976
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(12410..12414,1..1063)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
ORIGIN
        1 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
       61 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
      121 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
      181 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
      241 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
      301 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
      361 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
      421 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
      481 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
      541 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
      601 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
      661 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
      721 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
      781 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
      841 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
      901 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
      961 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     1021 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     1081 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac
     1141 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     1201 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     1261 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     1321 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     1381 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     1441 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     1501 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     1561 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca
     1621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     1681 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     1741 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1801 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2641 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     2701 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     2761 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     2821 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     2881 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     2941 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     3001 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     3061 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     3121 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     3181 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     3241 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     3301 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     3361 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     3421 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
     3481 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
     3541 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
     3601 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
     3661 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     3721 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     3781 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     3841 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     3901 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
     3961 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
     4021 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
     4081 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
     4141 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga
     4201 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     4261 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     4321 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     4381 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
     4441 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     4501 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     4561 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     4621 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     4681 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     4741 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     4801 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     4861 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     4921 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
     4981 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     5041 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     5101 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa
     5161 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg
     5221 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
     5281 cttccttata tagaggaagg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
     5341 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     5401 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     5461 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     5521 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     5581 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     5641 tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg
     5701 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
     5761 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
     5821 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
     5881 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
     5941 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
     6001 tgtcgactat cggacgagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
     6061 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
     6121 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
     6181 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
     6241 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
     6301 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
     6361 caggtatttc cgttggatac gaattattcg tacgaccctc ggcgcgccga gaacttccag
     6421 agctttgggt acgtcacctg atagtttaaa cggtccccag atttgccttt tcaatttcag
     6481 aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtctca tcaagacgat
     6541 ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt
     6601 caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag
     6661 aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga
     6721 gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca
     6781 aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt
     6841 acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta
     6901 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
     6961 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
     7021 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
     7081 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     7141 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
     7201 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     7261 ataaggaagt tcatttcatt tggagagaac acgggggact ctagaatggt gagtaaagga
     7321 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     7381 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     7441 aaatttattt gcactactgg aaaactacct gttccatggc caaccctggt caccaccctg
     7501 acctacggcg tgcagtgctt ctcccgttac cctgatcata tgaagcggca cgacttcttc
     7561 aagagcgcca tgcctgaggg atacgtgcag gagaggacca tcttcttcaa ggacgacggg
     7621 aactacaaga cacgtgctga agtcaagttt gagggagaca ccctcgtcaa caggatcgag
     7681 cttaagggaa tcgatttcaa ggaggacgga aacatcctcg gccacaagtt ggaatacaac
     7741 tacaactccc acaacgtata catcatggcc gacaagcaaa agaacggcat caaagccaac
     7801 ttcaagaccc gccacaacat cgaagacggc ggcgtgcaac tcgctgatca ttatcaacaa
     7861 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     7921 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     7981 acagctgctg ggattacaca tggcatggat gaactataca aataaagctc gaatttcccc
     8041 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     8101 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     8161 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     8221 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     8281 atgttactag atcgtgggcc cagctccaga cgtcgcggtg gtggctcgag cgcgtagtcc
     8341 tcggtaatat cgcagaacaa aagtacctga tatcgagtgt acttcaagtc agtgggaaat
     8401 caataaaatg attattttat gaatatattt cattgtgcaa gtagatagaa attacatatg
     8461 ttacataaca cacgaaataa acaaaaaaag acaatccaaa aacaaacacc ccaaaaaaaa
     8521 taatcacttt agataaactc gtatgaggag aggcacgttc agtgactcga cgattcccga
     8581 gcaaaaaaag tctccccgtc acacatgtag tgggtgacgc aattatcttt aaagtaatcc
     8641 ttctgttgac ttgtcattga taacatccag tcttcgtcag gattgcaaag aattatagaa
     8701 gggatcccac cttttatttt cttctttttt ccatatttag ggttgacagt gaaatcagac
     8761 tggcaaccta ttaattgctt ccacaatggg acgaacttga aggggatgtc gtcgatgata
     8821 ttataggtgg cgtgttcatc gtagttggtg aaatcgatgg taccgttcca atagttgtgt
     8881 cgtccgagac ttctagccca ggtggtcttt ccggtacgag ttggtccgca gatgtagagg
     8941 ctggggtgtc ggattccatt ccttccattg tccttgttaa atcggccatc cattcaaggt
     9001 cagattgagc ttgttggtat gagacaggat gtatgtaagt ataagcgtct atgcttacat
     9061 ggtatagatg ggtttccctc caggagtgta gatcttcgtg gcagcgaaga tctgattctg
     9121 tgaagggcga cacatacggt tcaggttgtg gagggaataa tttgttggct gaatattcca
     9181 gccattgaag ctttgttgcc cattcatgag ggaattcttc cttgatcatg tcaagatatt
     9241 cctccttaga cgttgcagtc tggataatag ttctccatcg tgcgtcagat ttgcgaggag
     9301 aaaccttatg atctcggaaa tctcctctgg ttttaatatc tccgtccttt gatatgtaat
     9361 caaggacttg tttagagttt ctagctggct ggatattagg gtgatttcct tcaaaatcga
     9421 aaaaagaagg atccctaata caaggttttt tatcaagctg gagaagagca tgatagtggg
     9481 tagtgccatc ttgatgaagc tcagaagcaa caccaaggaa gaaaataaga aaaggtgtga
     9541 gtttctccca gagaaactgg aataaatcat ctctttgaga tgagcacttg ggataggtaa
     9601 ggaaaacata tttagattgg agtctgaagt tcttactagc agaaggcatg ttgttgtgac
     9661 tccgaggggt tgcctcaaac tctatcttat aaccggcgtg gaggcatgga ggcaggggta
     9721 ttttggtcat tttaatagat agtggaaaat gacgtggaat ttacttaaag acgaagtctt
     9781 tgcgacaagg gggggcccac gccgaattta atattaccgg cgtggccccc ccttatcgcg
     9841 agtgctttag cacgagcggt ccagatttaa agtagaaaat ttcccgccca ctagggttaa
     9901 aggtgttcac actataaaag catatacgat gtgatggtat ttgatggagc gtatattgta
     9961 tcaggtattt ccgttggata cgaattattc gtacgaccct catagtttaa actatcagtg
    10021 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataacgga
    10081 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
    10141 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
    10201 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
    10261 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
    10321 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
    10381 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
    10441 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
    10501 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
    10561 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
    10621 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
    10681 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
    10741 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
    10801 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
    10861 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
    10921 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
    10981 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
    11041 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
    11101 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacggct caaccgtgcg
    11161 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
    11221 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
    11281 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
    11341 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
    11401 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
    11461 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
    11521 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
    11581 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
    11641 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
    11701 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
    11761 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
    11821 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
    11881 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
    11941 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
    12001 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
    12061 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
    12121 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
    12181 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
    12241 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
    12301 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
    12361 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcg
//
