LOCUS       sequence-78798-e         806 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  gateway adapted mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang 
            X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, 
            Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz
            G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, 
            Meyerson M
  TITLE     Functional analysis of receptor tyrosine kinase mutations in lung 
            cancer identifies oncogenic extracellular domain mutations of ERBB2.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug
            20.
  PUBMED    22908275
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-4"; volume: "109(36)"; pages: 
            "14476-81. Epub 2012 Aug 20"
COMMENT     Sequence Label: pBABEpuro-gateway
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..129
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(154..159,160..177,178..184)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(304..323)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 catcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
       61 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
      121 gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      181 aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
      241 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      301 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      361 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      421 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
      481 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
      541 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
      601 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
      661 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
      721 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
      781 aaggtgctga tgccgctggc gattca
//
