LOCUS       sequence-79177-e        8311 bp    DNA     circular SYN 14-APR-2025
DEFINITION  plasmid expresses FLAG/HA-tagged MOV10 D645N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Gregersen LH, Schueler M, Munschauer M, Mastrobuoni G, Chen W, Kempa
            S, Dieterich C, Landthaler M
  TITLE     MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target 
            degradation by translocation along 3' UTRs.
  JOURNAL   Mol Cell. 2014 May 22;54(4):573-85. doi: 
            10.1016/j.molcel.2014.03.017. Epub 2014 Apr 10.
  PUBMED    24726324
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.017"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "573-85"
COMMENT     Sequence Label: pFRT/FLAG/HA MOV10 D645N
FEATURES             Location/Qualifiers
     source          1..8311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             67..108
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(146..163)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    152..376
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(509..528)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    660..707
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             715..1737
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             715..1737
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1867..1988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1904..1923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1958..1977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2037..2053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2037..2053)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2050..2072)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2061..2077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2085..2091,2092..2109,2110..2115))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2130..2151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2268..2285)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2439..3027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2519..2538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3198..3989,3990..4058))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3821..3840
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4059..4163)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4238..4257)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4429..4808
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4809..5012
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4963..4983
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5057..5076
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5057..5075
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5140..5149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5149..5172
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5173..5199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5215..5239
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7888..7899
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(join(8301..8311,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct caagtctaga gggcccgcgg
       61 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgct cgagtctaga
      121 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
      181 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
      241 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
      301 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
      361 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
      421 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      481 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      541 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      601 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
      661 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
      721 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
      781 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
      841 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
      901 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
      961 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     1021 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     1081 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     1141 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     1201 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     1261 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     1321 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     1381 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     1441 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     1501 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     1561 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     1621 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     1681 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     1741 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     1801 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1861 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1921 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1981 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     2041 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2101 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2161 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2221 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2281 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2341 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2401 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2461 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2521 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2581 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2641 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2701 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2761 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2821 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2881 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2941 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3001 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3061 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3121 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3181 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3241 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3301 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3361 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3421 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3481 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3541 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3601 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3661 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3721 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3781 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3841 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3901 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3961 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4021 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4081 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4141 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     4201 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     4261 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     4321 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     4381 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     4441 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     4501 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     4561 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     4621 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     4681 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     4741 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4801 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4861 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4921 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4981 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
     5041 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagcgagctc
     5101 ggatcgatcc atcgatagtg tggtggaatt ctgatcgccg ccgccatgga ctacaaggac
     5161 gacgatgaca agtaccctta tgacgtgccc gattacgctt tgaaccagat atcaacaagt
     5221 ttgtacaaaa aagcaggctc caccatggga accaattcag tcgacatgcc cagtaagttc
     5281 agctgccggc agctccggga ggcgggccag tgtttcgaga gtttcctggt cgttcgggga
     5341 ctggacatgg agacagatcg cgagcggctg cggaccattt ataaccgcga cttcaagatc
     5401 agctttggga cccccgcccc tggcttctcc tccatgctgt atggaatgaa gattgcaaat
     5461 ctggcctacg tcaccaagac tcgggtcagg ttcttcagac tcgaccgctg ggccgacgtg
     5521 cggttcccag aaaagaggag aatgaagctg gggtcagata tcagcaaaca ccacaagtca
     5581 ctgctagcca agatctttta tgacagggct gagtatcttc atgggaaaca tggtgtggat
     5641 gtggaagtcc aggggcccca tgaagcccga gatgggcagc tccttatccg cctggatttg
     5701 aaccgcaaag aggtgctgac cctgaggctt cggaatggcg gaacccagtc tgttaccctc
     5761 actcacctct tcccactctg ccggacaccc cagtttgctt tctacaatga agaccaggag
     5821 ttgccctgtc cactgggccc cggtgaatgc tatgaactcc atgtccattg taagaccagc
     5881 tttgtgggct acttcccagc cacagtgctc tgggagctgc tgggacctgg ggagtcgggt
     5941 tcagaaggag ccggcacatt ctacattgcc cgcttcttgg ctgccgtcgc ccacagcccc
     6001 ctggctgcac agctgaagcc catgactccc ttcaagcgga cccggatcac cggaaaccct
     6061 gtggtgacca atcggataga ggaaggagag agacctgacc gcgctaaggg ctatgacctg
     6121 gagttaagta tggcgctggg gacatactac ccacctcccc gcctcaggca gctgctcccc
     6181 atgcttcttc agggaacaag tatcttcact gcccctaagg agatcgcaga gatcaaggcc
     6241 cagctggaga cagccctgaa gtggaggaac tatgaggtga agctgcggct gctgctgcac
     6301 ctggaggaac tgcagatgga gcatgatatc cggcactatg acctggagtc ggtgcccatg
     6361 acctgggacc ctgtggacca gaaccccagg ctgctcacgc tggaggttcc tggagtgact
     6421 gagagccgcc cctcagtgct acggggcgac cacctgtttg cccttttgtc ctcggagaca
     6481 caccaggagg accccatcac atataagggc tttgtgcaca aggtggaatt ggaccgtgtc
     6541 aagctgagct tttccatgag cctcctgagc cgctttgtgg atgggctgac cttcaaggtg
     6601 aactttacct tcaaccgcca gccgctgcga gtccagcacc gtgccctgga gctgacaggg
     6661 cgctggctgc tgtggcccat gctctttcct gtggcacctc gggacgtccc gctgctgccc
     6721 tcagatgtga aactcaagct gtacgaccgg agtctggagt caaacccaga gcagctgcag
     6781 gccatgaggc acattgttac gggcaccacc cgtccagccc cctacatcat ctttgggcct
     6841 ccaggcaccg gcaagactgt cacgttagtg gaggcaatta agcaggtggt gaagcacttg
     6901 cccaaagccc acatcttggc ctgcgctcca tccaactcag gggctgacct actctgtcaa
     6961 aggctccggg tccaccttcc tagctccatc taccgcctcc tggcccccag cagggacatc
     7021 cgcatggtac ctgaggacat caagccctgc tgcaactggg acgcaaagaa gggggagtat
     7081 gtatttcccg ccaagaagaa gctgcaggaa taccgggtct taattaccac cctcatcact
     7141 gccggcaggt tggtctcggc ccagtttccc attgatcact tcacacacat cttcatcaat
     7201 gaggctggcc actgcatgga gcctgagagt ctggtagcta tagcagggct gatggaagta
     7261 aaggaaacag gtgatccagg agggcagctg gtgctggcag gagaccctcg gcagctgggg
     7321 cctgtgctgc gttccccact gacccagaag catggactgg gatactcact gctggagcgg
     7381 ctgctcacct acaactccct gtacaagaag ggccctgatg gctatgaccc ccagttcata
     7441 accaagctgc tccgcaacta caggtctcat cccaccatcc tggacattcc taaccagctc
     7501 tattatgaag gggagctgca ggcctgtgct gatgtcgtgg atcgagaacg cttctgccgc
     7561 tgggcgggcc tacctcgaca gggctttccc atcatctttc acggcgtaat gggcaaagat
     7621 gagcgtgaag gcaacagccc atccttcttc aaccctgaag aggctgccac agtgacttcc
     7681 tacctgaagc tgctcctggc cccctcctcc aagaagggca aagctcgcct gagccctcga
     7741 agtgtgggcg tcatctcccc gtaccggaaa caggtggaga aaatccgtta ctgcatcacc
     7801 aaacttgaca gggagcttcg aggactggat gacatcaagg acttgaaggt gggttcagta
     7861 gaagaattcc aaggccaaga acgaagcgtc atcctcatct ccaccgtgcg aagcagccag
     7921 agctttgtgc agctggatct ggactttaat ctgggtttcc ttaagaaccc caagaggttc
     7981 aatgtagctg tgacccgggc caaggccctg ctcatcatcg tggggaaccc ccttctcctg
     8041 ggccatgacc ctgactggaa agtattcctg gagttctgta aagaaaacgg agggtatacc
     8101 gggtgtccct tccctgccaa actggacctg caacagggac agaatttact gcaaggtctg
     8161 agcaagctca gcccctctac ctcagggccc cacagccatg actacctccc ccaggagcgg
     8221 gagggtgaag ggggcctgtc tctgcaagtg gagccagagt ggaggaatga gctctgagcg
     8281 gccgcactcg agatatctag acccagcttt c
//
