LOCUS       sequence-79514-e         878 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon 
            configuration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-FAS
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             147..191
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     229..248
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        229..247
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    248..272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             287..309
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ataccagcgc tgcgtctgtg ccgtgcgctc tggatgaagc ggttcgtgac ggtcgcatta
       61 aaccgggcca gctggtcctg ctggaagcgt tcggcggtgg ttttacctgg ggttctgctc
      121 tggtgcgttt ttaactcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc
      181 acatggactc gtctactagg atcgagatcg atctcgatcc cgcgaaatta atacgactca
      241 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      301 agaaggagat atacataata caggcggcat atgaattttg aaggaaaaat cgcactggta
      361 accggtgcaa gccgcggaat tggccgcgca attgctgaaa cgctcgcagc ccgtggcgcg
      421 aaagttattg gcactgcgac cagtgaaaat ggcgctcagg cgatcagtga ttatttaggt
      481 gccaacggca aaggtctgat gttgaatgtg accgacccgg catctatcga atctgttctg
      541 gaaaaaattc gcgcagaatt tggtgaagtg gatatcctgg tcaataatgc cggtatcact
      601 cgtgataacc tgttaatgcg aatgaaagat gaagagtgga acgatattat cgaaaccaac
      661 ctttcatctg ttttccgtct gtcaaaagcg gtaatgcgcg ctatgatgaa aaagcgtcat
      721 ggtcgtatta tcactatcgg ttctgtggtt ggtaccatgg gaaatggcgg tcaggccaac
      781 tacgctgcgg cgaaagcggg cttgatcggc ttcagtaaat cactggcgcg cgaagttgcg
      841 tcacgcggta ttactgtaaa cgttgttgct ccgggctt
//
