LOCUS       sequence-79628-e         894 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  expresses human Flag tagged KLF6 with 4D mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Lang UE, Kocabayoglu P, Cheng GZ, Ghiassi-Nejad Z, Munoz U, Vetter 
            D, Eckstein DA, Hannivoort RA, Walsh MJ, Friedman SL
  TITLE     GSK3beta phosphorylation of the KLF6 tumor suppressor promotes its 
            transactivation of p21.
  JOURNAL   Oncogene. 2012 Oct 22. doi: 10.1038/onc.2012.457.
  PUBMED    23085750
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2012 Oct 22. doi: 10.1038/onc.2012.457."
COMMENT     Sequence Label: Flag-KLF6-4D (1054)
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14..37
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggccgccacc atggattaca aggacgacga cgataagatc tgtcgacggt accccgggga
       61 attcgccctt gggctccgtc caacccggcc cgacatggac gtgctcccca tgtgcagcat
      121 cttccaggag ctccagatcg tgcacgagac cggctacttc tcggcgctgc cgtctctgga
      181 ggagtactgg caacagacct gcctagagct ggaacgttac ctccagagcg agccctgcta
      241 tgtttcagcc tcagaaatca aatttgacag ccaggaagat ctgtggacca aaatcattct
      301 ggctcgggag aaaaaggagg aatccgaact gaagatatct tccagtcctc cagaggacac
      361 tctcatcagc ccgagctttt gttacaactt agagaccaac agcctgaact cagatgtcag
      421 cagcgaatcc tctgacagct ccgaggaact ttctcccacg gccaagttta cctccgaccc
      481 cattggcgaa gttttggtca gctcgggaaa attgagctcc gatgtcacct ccgatcctcc
      541 atctgatccg gaactggaca gggaaccttc tcaactgtgg ggttgcgtgc ccggggagct
      601 gccctcgcca gggaaggtgc gcagcgggac ttcggggaag ccaggtgaca agggaaatgg
      661 cgatgcctcc cccgacggca ggaggagggt gcaccggtgc cactttaacg gctgcaggaa
      721 agtttacacc aaaagctccc acttgaaagc acaccagcgg acgcacacag gagaaaaagc
      781 cttacagatg ctcatgggga agggtgtgag tggcgttttg caagaagtga tgagttaacc
      841 aggcacttcc gaaagcacac cggggccaag ccttttaaat gctcccactg tgac
//
