LOCUS       sequence-79778-e         983 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  expression of EGFP-tagged COOH-terminal AA 661–793 KAP3B in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Brown CL, Maier KC, Stauber T, Ginkel LM, Wordeman L, Vernos I, 
            Schroer TA
  TITLE     Kinesin-2 is a motor for late endosomes and lysosomes.
  JOURNAL   Traffic. 2005 Dec;6(12):1114-24.
  PUBMED    16262723
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic."; 
            date: "2005-12"; volume: "6(12)"; pages: "1114-24"
COMMENT     Sequence Label: KAP3-CT
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(53..55,56..58,59..769)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(53..55,56..58,59..769)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(98..119)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(359..378)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     706..727
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag
       61 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      121 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      181 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      241 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      301 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      361 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      421 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      481 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      541 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      601 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      661 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      721 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt actcagatct
      781 cgaggaatat gatgaagaat gggctaagaa aattcagagt gaaaagtttc gctggcataa
      841 ctctcagtgg ctggagatgg tagagagtcg tcagatggat gagagtgagc agtacttgta
      901 tggtgatgat cgaattgagc catacattca tgaaggagat attctcgaaa gacctgacct
      961 tttctacaac tcagatggat taa
//
