LOCUS       sequence-79815-e         879 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses human NKCC1 with truncation of N-terminus, mutations at 
            P676C I730C and C-terminus replaced with that of NBCe. Contains an 
            N-terminal 3xFLAG-YFP tag for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-termina...
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gagctctaca agggtgacct ggagccgagg cccacggctc ctagctccgg cgcgcgcgcc
       61 aggatcgacc actaccggca cacagccgcg cagctcggcg agaagctgct ccggcctagc
      121 ctggccgagc tgcatgacga gctggaaaag gaaccttttg aggatggctt tgcaaatggg
      181 gaagaaagca ctccaacacg tgatgctgtg gtcacgtaca cggccgaaag taaaggagtc
      241 gtgaagtttg gctggatcaa gggtgtacta gtacgttgta tgttaaacat ttggggtgtg
      301 atgcttttca ttcgtctctc atggattgtg ggacaagctg gaattggtct atcagtcctt
      361 gtaataatga tggctactgt tgtgacaact atcacaggat tgtccactag cgccatcgca
      421 actaatggat ttgtaagggg aggcggcgca tattatttaa tatctagaag tctagggcct
      481 gaatttggtg gtgccattgg tctaatcttc gcctttgcca acgctgttgc tgttgctatg
      541 tatgtggttg gatttgcaga aaccgtggtg gagttgctga aagaacattc cattcttatg
      601 atagatgaaa tcaatgacat ccggattatt ggagccatta cagtcgtgat tcttttaggt
      661 atctcagtag ccggtatgga gtgggaagca aaagctcaga ttgttctttt ggtgatccta
      721 cttcttgcta ttggtgattt cgtcatagga acatttatcc cactcgagag caagaagcca
      781 aaagggtttt ttggctataa atctgaaatc tttaatgaga actttggccc cgattttcga
      841 gaagaagaaa ctttcttttc tgtatttgcc atctttttt
//
