LOCUS       sequence-80013-e        1696 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  mammalian expression of Nano-lantern, a luminescent protein for 
            high-speed single-cell and whole body imaging, targeted to the 
            plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern-plasma membrane/pcDNA3
FEATURES             Location/Qualifiers
     source          1..1696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(71..73,74..76,77..589)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(116..137)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(377..396)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     724..745
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgggctgca tcaagagcaa gcgcaaggac aacctgaacg acgacggcgt ggacatgaag
       61 accggatccg atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      121 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      181 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc
      241 ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga
      301 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      361 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      421 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      481 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa
      541 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
      601 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      661 cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga
      721 tcacatggtc ctgctggagt tcgtgaccgc cgccgggggt accaaggtgt acgaccccga
      781 gcagaggaag aggatgatca ccggccccca gtggtgggcc aggtgcaagc agatgaacgt
      841 gctggacagc ttcatcaact actacgacag cgagaagcac gccgagaacg ccgtgatctt
      901 cctgcacggc aacgccacta gcagctacct gtggaggcac gtggtgcccc acatcgagcc
      961 cgtggccagg tgcatcatcc ccgatctgat cggcatgggc aagagcggca agagcggcaa
     1021 cggcagctac aggctgctgg accactacaa gtacctgacc gcctggttcg agcttctgaa
     1081 cctgcccaag aagatcatct tcgtgggcca cgactggggc gccgccctgg ccttccacta
     1141 cgcctacgag caccaggaca ggatcaaggc catcgtgcac atggagagcg tggtggacgt
     1201 gatcgagagc tgggacgagt ggccagacat cgaggaggac atcgccctga tcaagagcga
     1261 ggagggcgag aagatggtgc tggagaacaa cttcttcgtg gagaccgtgc tgcccagcaa
     1321 gatcatgaga aagctggagc ccgaggagtt cgccgcctac ctggagccct tcaaggagaa
     1381 gggcgaggtg agaagaccca ccctgagctg gcccagagag atccccctgg tgaagggcgg
     1441 caagcccgac gtggtgcaga tcgtgagaaa ctacaacgcc tacctgagag ccagcgacga
     1501 cctgcccaag ctgttcatcg agggcgaccc cggcttcttc agcaacgcca tcgtggaggg
     1561 cgccaagaag ttccccaaca ccgagttcgt gaaggtgaag ggcctgcact tcctccagga
     1621 ggacgccccc gacgagatgg gcaagtacat caagagcttc gtggagagag tgctgaagaa
     1681 cgagcagtaa ctcgag
//
