LOCUS       sequence-80061-e         876 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  T-DNA destination plasmid. Contains the bean yellow dwarf virus 
            cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. 
            Contains attR1 and attR2 sites between the LIR and SIR sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSL
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(131..255)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
       61 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
      121 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
      181 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
      241 cttgtacaaa gtggtgagtg tacttcaagt cagtgggaaa tcaataaaat gattatttta
      301 tgaatatatt tcattgtgca agtagataga aattacatat gttacataac acacgaaata
      361 aacaaaaaaa gacaatccaa aaacaaacac cccaaaaaaa ataatcactt tagataaact
      421 cgtatgagga gaggcacgtt cagtgactcg acgattcccg agcaaaaaaa gtctccccgt
      481 cacacatgta gtgggtgacg caattatctt taaagtaatc cttctgttga cttgtcattg
      541 ataacatcca gtcttcgtca ggattgcaaa gaattataga agggatccca ctcgagccaa
      601 gcttggcgtg tctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata
      661 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
      721 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
      781 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
      841 atgcctctgc cgacagtggt cccaaagatg gacccc
//
