LOCUS       sequence-80067-e         604 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  T-DNA destination plasmid. Contains the bean yellow dwarf virus 
            cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. 
            Contains attR1 and attR2 sites between the LIR and SIR sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSL
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(154..278)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta
      181 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      241 ttttacgttt ctcgttcagc ttttttgtac aaacttgtag aaatgattta aattgctgca
      301 atagaagtag aatgcttgat tgcttgagat tcgtttgttt tgtatatgtt gtgttgagaa
      361 ctctcgacgt cctgcaaaca atacacaaca aagacaatga gatttggttt tataaacaat
      421 caagaatctt agggagggtc gtacgaataa ttcgtatcca acggaaatac ctgatacaat
      481 atacgctcca tcaaatacca tcacatcgta tatgctttta tagtgtgaac acctttaacc
      541 ctagtgggcg ggaaattttc tactttaaat ctggaccgct cgtgctaaag cactcgcgat
      601 aagg
//
