LOCUS       sequence-80253-e         876 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Neuronal expression of GCaMP6s Ca sensor and physically separate 
            nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV-hSyn1-GCaMP6s-P2A-nls-dTomato
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..102
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(106..114,115..129)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      136..145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             157..213
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     426..447
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(553..574)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(814..833)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcgcagtcg agaaaccggc tagaggatcc ggcgccacca tgggttctca tcatcatcat
       61 catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat
      121 gacgataagg atctcgccac catggtcgac tcatcacgtc gtaagtggaa taagacaggt
      181 cacgcagtca gagctatagg tcggctgagc tcactcgaga acgtctatat caaggccgac
      241 aagcagaaga acggcatcaa ggcgaacttc cacatccgcc acaacatcga ggacggcggc
      301 gtgcagctcg cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      361 cccgacaacc actacctgag cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc
      421 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      481 ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg
      541 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      601 ggcgagggtg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      661 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      721 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      781 ggctacatcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      841 gaggtgaagt tcgagggcga caccctggtg aaccgc
//
