LOCUS       sequence-80587-e         510 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Supplies the selection marker (hygromycin resistance) and IRES with 
            flanked by AttR2 and AttL3 sites for Gateway cloning recombination 
            reaction. (This corresponds to a module 3 plasmid)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-iHygro-L3   #BGV206
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    195..216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    271..287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     276..298
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     295..311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     295..311
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(342..466)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt
       61 tgccgcggcc ctctcacttc cctcgcgaaa gctccaatac gcaaaccgcc tctccccgcg
      121 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      181 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      241 atgcttccgc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      301 acagctatga ccatgattac gccactagca gatctgccgg ccatagtgac tggatatgtt
      361 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
      421 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgcta gcgcggccgc
      481 ccccttcacc ttggatccgc cggcgaattc
//
