LOCUS       sequence-80701-e         808 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  p2attNG landing pad plasmid with 5 and 3kb homology arms to the 
            human H11 locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attNG-H11-long
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..187
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 agttcctatt cttcaaaagg tataggaact tcaagcttct aaccccgcat accaagaaac
       61 cccctacccg gcccgcgaag gctaggtagg gggctttttg tgtttcagtg ggtatggccg
      121 tgatgacctg tgtcttcgtg gtttgtctgg tcaaccaccg cggtctcagt ggtgtacggt
      181 acaaacccat gagagccctg gtagtcatcc gcctgtcccg cgtcaccgat gctacgactt
      241 caccggagcg tcagctggag tcttgccagc agctctgcgc ccagcgcggc ggccgcctag
      301 taaggggcgc gccccttttt ccttgagctt taaagacccc aacaggtcag tttatactgg
      361 actgacccta tagatagcat agccttgtgg ctaataccag gatatctata gtgtaatttc
      421 caagattaaa ctgacctagt gaaaacagtt tattgaggaa tcatctatta catatggtag
      481 tttctctcaa acaaatcatg tttactgggg catcctgttt ctctaagctc agagttctga
      541 gcttagaccc actgaagtct aagaatttcc cttaagactc atgcattaat tgactccttg
      601 tgttctgtat cacattcctt agctcacaat tgttttatta ttcaccttaa ctattagtat
      661 atacatagca cttacggata aagattctac agaatacagt acagttctga gtcccctttc
      721 ctaactggag gtttttcagt cctgctatga ggtgatactt atttcccatg ctactcaagt
      781 taatttacca gagttctaga tatttgtc
//
