LOCUS       sequence-80801-e         972 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Stage-Zimmermann T, Schmidt U, Silver PA
  TITLE     Factors affecting nuclear export of the 60S ribosomal subunit in 
            vivo.
  JOURNAL   Mol Biol Cell 2000 Nov;11(11):3777-89.
  PUBMED    11071906
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2000-11"; volume: "11(11)"; pages: "3777-89"
COMMENT     Sequence Label: pPS2167
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(348..366)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(387..403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(387..403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(400..422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    411..427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(435..441,442..459,460..465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    480..501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(618..635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(869..888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catggcatgg atgaactata caaaggatcc ggctgctaac aaagcccgaa aggaagcttc
       61 ggtatactta tttttactcc cccttctgtg ttttcttctt tttttttgtg tttctttcgt
      121 ggctgtttct taatcttata cgtaccttta tctatttccg tacagttgta atctatcttc
      181 tttagatctt gccatttttt atgcggtata tcccaatggc attttttagc tacgagactg
      241 gccacgtcat cacagaatcc caacaggttt aagtcaaact ctgcatgtgt aaccatatcc
      301 ctattgatca aaatctgcgg aacatgtgaa ggtaccagct tttgttccct ttagtgaggg
      361 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      421 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      481 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      541 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      601 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      661 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      961 tcgggaagcg tg
//
