LOCUS       sequence-80818-e        8898 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Bacterial expression of mouse TDG C-terminally fused to GyrA intein,
            SUMO-E2 and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-mTDG-IntE2
FEATURES             Location/Qualifiers
     source          1..8898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1361..1954
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             2471..3127
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(3176..3194)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3190..3237
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3297..3401
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3402..3470,3471..4262)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3620..3639)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4304..4817)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4499..4520)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4711..4730
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4928..5516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5417..5436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5670..5687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5709..5728)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5869..5891
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5885..6076)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6633..7715)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7677..7696
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7716..7793)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      7946..8032
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8129..8215
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8312..8398
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8495..8581
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8678..8764
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataaccat ggacgcagag gccgcgcgca gctattctct
      121 ggagcaagtt caagctttgt attcatttcc atttcaacaa atgatggcag aagttcctaa
      181 catggcagtc acgactggac agcaggtgcc agcagtagct cctaacatgg caaccgtgac
      241 agaacagcag gtgcccgcag acgctcctgt ccaggaacct gcaccagaag ctccaaagag
      301 aaggaaaagg aaacccagag cagcagagcc ccaggaacca gtggagccca aaaaacctgc
      361 tacgtcgaag aaatccggca agtctacaaa atcaaaggaa aagcaggaga aaatcacaga
      421 cgcgtttaaa gtgaaaagga aagtggaccg cttcaacggc gtctctgaag ctgagcttct
      481 gaccaagact cttcctgaca ttttgacctt caatctggat attgtgatca ttggcattaa
      541 cccgggatta atggctgctt acaaaggaca tcactaccct gggcctggaa atcacttctg
      601 gaagtgtctg ttcatgtcgg ggctgagtga ggtgcagctg aatcacatgg atgaccacac
      661 cttacccggc aagtacggca tcggattcac caacatggtg gaacggacga cgccgggcag
      721 caaggatctg tctagtaaag agttccggga aggagggcgc atcctggtgc agaaactgca
      781 gaaatatcag ccacgaatag cggtgtttaa tggaaaatgt atttatgaaa ttttcagtaa
      841 agaagttttt ggagtaaagg ttaagaactt ggaatttggg cttcaacccc acaagatccc
      901 agacacagaa actctgtgct acgtcatgcc gtcgtccagc gccagatgtg ctcagtttcc
      961 ccgggcccag gacaaagttc attactacat taagctgaag gacttgagag accagctgaa
     1021 aggcattgaa cgcaacaccg acgttcagga agtgcagtat acatttgacc tgcagcttgc
     1081 gcaagaggac gcaaagaaga tggctgttaa ggaagaaaag tatgatccag gctatgaggc
     1141 agcttacggc ggcgcctatg gggaaaaccc atgtaatggg gaaccttgtg gcattgcttc
     1201 aaatgggcta acagcccaca gtgcggagcc gagaggagaa gcgacccccg gcgatgttcc
     1261 aaatgggcag tggatggcac agtcgtttgc agagcagatc ccttctttta ataattgtgg
     1321 gacccgagag caggaagaag agagccacgc tctcgagatg tgcatctgcg gagatgcact
     1381 agttgcccta cccgagggcg agtcggtacg catcgccgac atcgtgccgg gtgcgcggcc
     1441 caacagtgac aacgccatcg acctgaaagt ccttgaccgg catggcaatc ccgtgctcgc
     1501 cgaccggctg ttccactccg gcgagcatcc ggtgtacacg gtgcgtacgg tcgaaggtct
     1561 gcgtgtgacg ggcaccgcga accacccgtt gttgtgtttg gtcgacgtcg ccggggtgcc
     1621 gaccctgctg tggaagctga tcgacgaaat caagccgggc gattacgcgg tgattcaacg
     1681 cagcgcattc agcgtcgact gtgcaggttt tgcccgcggg aaacccgaat ttgcgcccac
     1741 aacctacaca gtcggcgtcc ctggactggt gcgtttcttg gaagcacacc accgagaccc
     1801 ggacgcccaa gctatcgccg acgagctgac cgacgggcgg ttctactacg cgaaagtcgc
     1861 cagtgtcacc gacgccggcg tgcagccggt gtatagcctt cgtgtcgaca cggcagacca
     1921 cgcgtttatc acgaacgggt tcgtcagcca cgctactggc ctcaccggtc taagcttagc
     1981 atccatgtcg gggatcgccc tcagcagact cgcccaggag aggaaagcat ggaggaaaga
     2041 ccacccattt ggtttcgtgg ctgtcccaac aaaaaatccc gatggcacga tgaacctcat
     2101 gaactgggag tgcgccattc cgggaaagaa agggactccg tgggaaggag gcttgtttaa
     2161 actacggatg cttttcaaag atgattatcc atcttcgcca ccaaaatgta aattcgaacc
     2221 accattattt cacccgaatg tgtacccttc ggggacagtg tgcctgtcca tcttagagga
     2281 ggacaaggac tggaggccag ccatcacaat caaacagatc ctattaggaa tacaggaact
     2341 tctaaatgaa ccaaatatcc aagacccagc tcaagcagag gcctacacga tttactgcca
     2401 aaacagagtg gagtacgaga aaagggtccg agcacaagcc aagaagtttg cgccctcatc
     2461 aggatccgga atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac
     2521 tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga
     2581 aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta
     2641 ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga
     2701 caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg
     2761 agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac
     2821 tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt
     2881 atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga
     2941 cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt
     3001 ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa
     3061 gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc
     3121 aaaatagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     3181 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     3241 aaaggaggaa ctatatccgg ataactacgt caggtggcac ttttcgggga aatgtgcgcg
     3301 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3361 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3421 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3481 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3541 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tctccaatga
     3601 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     3661 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3721 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3781 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3841 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3901 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3961 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4021 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4081 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4141 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4201 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4261 aactgtcaga ccaagtttac tcatatatac tttagattga tttaccccgg ttgataatca
     4321 gaaaagcccc aaaaacagga agattgtata agcaaatatt taaattgtaa acgttaatat
     4381 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4441 aatcggcaaa atcccttata aatcaaaaga atagcccgag atagggttga gtgttgttcc
     4501 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4561 cgtctatcag ggcgatggcc cactacgtga accatcaccc aaatcaagtt ttttggggtc
     4621 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4681 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4741 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4801 gccgctacag ggcgcgtaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4861 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4921 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4981 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5041 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     5101 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5161 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5221 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5281 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5341 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5401 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5461 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5521 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5581 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5641 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gctatggtgc
     5701 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     5761 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     5821 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5881 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     5941 cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     6001 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     6061 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     6121 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     6181 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     6241 tcactcaggg tcaatgccag ccgaacgcca gcaagacgta gcccagcgcg tcggccgcca
     6301 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg
     6361 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc
     6421 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga
     6481 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
     6541 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat
     6601 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6721 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
     6781 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
     6841 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     6901 cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     6961 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
     7021 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
     7081 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
     7141 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
     7201 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
     7261 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
     7321 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
     7381 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
     7441 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
     7501 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
     7561 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     7621 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     7681 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct
     7741 tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc
     7801 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     7861 gttgagcacc gccgccgcaa ggaatggtgc atgccggcat gccgcccttt cgtcttcaag
     7921 aattaattcc caattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     7981 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     8041 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     8101 aactgccagg aattaattcc ccaggcatca aataaaacga aaggctcagt cgaaagactg
     8161 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     8221 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     8281 ataaactgcc aggaattaat tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     8341 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     8401 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     8461 gccataaact gccaggaatt aattccccag gcatcaaata aaacgaaagg ctcagtcgaa
     8521 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     8581 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     8641 cccgccataa actgccagga attaattccc caggcatcaa ataaaacgaa aggctcagtc
     8701 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     8761 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     8821 acgcccgcca taaactgcca ggaattgggg atcggaatta attcccggtt taaaccgggg
     8881 atctcgatcc cgcgaaat
//
