LOCUS       sequence-80922-e         917 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Park JE, Heo I, Tian Y, Simanshu DK, Chang H, Jee D, Patel DJ, Kim 
            VN
  TITLE     Dicer recognizes the 5' end of RNA for efficient and accurate 
            processing.
  JOURNAL   Nature. 2011 Jul 13;475(7355):201-5. doi: 10.1038/nature10198.
  PUBMED    21753850
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-07-13- 13"; volume: "475"; issue: "7355"; 
            pages: "201-5"
COMMENT     Sequence Label: pcDNA3.1-FLAG-Dicer-5'-pocket mutant
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             82..105
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcccaccat ggactacaag gacgacgatg acaagaagct tatggcaggc
      121 ctgcagctca tgacccctgc ttcctcacca atgggtcctt tctttggact gccatggcaa
      181 caagaagcaa ttcatgataa catttatacg ccaagaaaat atcaggttga actgcttgaa
      241 gcagctctgg atcataatac catcgtctgt ttaaacactg gctcagggaa gacatttatt
      301 gcagtactac tcactaaaga gctgtcctat cagatcaggg gagacttcag cagaaatgga
      361 aaaaggacgg tgttcttggt caactctgca aaccaggttg ctcaacaagt gtcagctgtc
      421 agaactcatt cagatctcaa ggttggggaa tactcaaacc tagaagtaaa tgcatcttgg
      481 acaaaagaga gatggaacca agagtttact aagcaccagg ttctcattat gacttgctat
      541 gtcgccttga atgttttgaa aaatggttac ttatcactgt cagacattaa ccttttggtg
      601 tttgatgagt gtcatcttgc aatcctagac cacccctatc gagaaattat gaagctctgt
      661 gaaaattgtc catcatgtcc tcgcattttg ggactaactg cttccatttt aaatgggaaa
      721 tgtgatccag aggaattgga agaaaagatt cagaaactag agaaaattct taagagtaat
      781 gctgaaactg caactgacct ggtggtctta gacaggtata cttctcagcc atgtgagatt
      841 gtggtggatt gtggaccatt tactgacaga agtgggcttt atgaaagact gctgatggaa
      901 ttagaagaag cacttaa
//
