LOCUS       sequence-81178-e        1086 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expression of truncated Mdm2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Oliver TG, Meylan E, Chang GP, Xue W, Burke JR, Humpton TJ, Hubbard 
            D, Bhutkar A, Jacks T
  TITLE     Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive 
            feedback loop.
  JOURNAL   Mol Cell. 2011 Jul 8;43(1):57-71. doi: 10.1016/j.molcel.2011.06.012.
  PUBMED    21726810
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.06"; journalName: "Mol Cell"; date: 
            "2011-07-8- 8"; volume: "43"; issue: "1"; pages: "57-71"
COMMENT     Sequence Label: pCMV-myc3-Mdm2 p60
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(257..276)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    322..377
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(376..395)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(735..751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(735..751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(748..770)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    759..775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(783..789,790..807,808..813))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    828..849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(966..983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaaatgaatc ccccccttcc atcacattgc aacagatgtt gggcccttcg tgagaattgg
       61 cttcctgaag ataaagggaa agataaaggg gaaatctctg agaaagccaa actggaaaac
      121 tcaacacaag ctgaagaggg ctttgatgtt cctgattagt aggatccgtc gaggaattca
      181 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
      241 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
      301 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
      361 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
      421 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
      481 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
      541 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
      601 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
      661 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ggatcggccg
      721 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      781 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      841 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      901 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      961 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1021 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1081 tgagca
//
