LOCUS       sequence-81202-e        1025 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  stable knockdown of Caspase-2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Oliver TG, Meylan E, Chang GP, Xue W, Burke JR, Humpton TJ, Hubbard 
            D, Bhutkar A, Jacks T
  TITLE     Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive 
            feedback loop.
  JOURNAL   Mol Cell. 2011 Jul 8;43(1):57-71. doi: 10.1016/j.molcel.2011.06.012.
  PUBMED    21726810
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.06"; journalName: "Mol Cell"; date: 
            "2011-07-8- 8"; volume: "43"; issue: "1"; pages: "57-71"
COMMENT     Sequence Label: MSCV-shCasp2-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           80..201
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           229..243
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           271..397
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        411..910
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(446..469)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     843..862
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(931..950)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat ctctcgacta
       61 gggataacag ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
      121 atcttggaaa cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag
      181 gtatattgct gttgacagtg agcgccccaa cttccctgtt ctttaatagt gaagccacag
      241 atgtattaaa gaacagggaa gttgggatgc ctactgcctc ggaattcaag gggctacttt
      301 aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga tacattttta
      361 caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattctacc gggtagggga
      421 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
      481 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
      541 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
      601 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
      661 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
      721 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
      781 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
      841 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
      901 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
      961 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     1021 cccgc
//
