LOCUS       sequence-81380-e       15384 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses nuclear mTagBFP and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15384)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 15384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-N-P-M-nucBFP-EGFP-L
FEATURES             Location/Qualifiers
     source          1..15384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4695..5393
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             5418..5438
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5442..5462
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5466..5486
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5571..5580
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5577..5579,5580..5582,5583..6296)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5577..5579,5580..5582,5583..6293)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5622..5643)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5883..5902)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6230..6251
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8045..8056
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13056..13074)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13070..13117
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13140..13156)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13140..13156)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13193..13213)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13193..13211)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13232..13248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13232..13248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13245..13267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13256..13272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13280..13286,13287..13304,13305..13310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13325..13346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13463..13480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13634..14222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13714..13733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14393..15184,15185..15253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15016..15035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15254..15358)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     4741 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     4801 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     4861 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     4921 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     4981 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     5041 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     5101 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     5161 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga
     5221 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     5281 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     5341 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aattccggac
     5401 tcagatctcg agctgatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg
     5461 tagatccaaa aaagaagaga aaggtaggat ccaccggatc tagataatta attaacgtcc
     5521 tttcaacgat ccaagtccat gaaaaaaact aacacccctc ccgtacggcc gccaccatgg
     5581 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     5641 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     5701 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5761 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     5821 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5881 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5941 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     6001 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     6061 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     6121 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6181 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     6241 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcta
     6301 gcttataaag tgctgggtca tctaagcttt tcagtcgaga aaaaaacatt agatcagaag
     6361 aacaactggc aacacttctc aacctgagac ttacttcaag atgctcgatc ctggagaggt
     6421 ctatgatgac cctattgacc caatcgagtt agaggctgaa cccagaggaa cccccattgt
     6481 ccccaacatc ttgaggaact ctgactacaa tctcaactct cctttgatag aagatcctgc
     6541 tagactaatg ttagaatggt taaaaacagg gaatagacct tatcggatga ctctaacaga
     6601 caattgctcc aggtctttca gagttttgaa agattatttc aagaaggtag atttgggttc
     6661 tctcaaggtg ggcggaatgg ctgcacagtc aatgatttct ctctggttat atggtgccca
     6721 ctctgaatcc aacaggagcc ggagatgtat aacagacttg gcccatttct attccaagtc
     6781 gtcccccata gagaagctgt tgaatctcac gctaggaaat agagggctga gaatcccccc
     6841 agagggagtg ttaagttgcc ttgagagggt tgattatgat aatgcatttg gaaggtatct
     6901 tgccaacacg tattcctctt acttgttctt ccatgtaatc accttataca tgaacgccct
     6961 agactgggat gaagaaaaga ccatcctagc attatggaaa gatttaacct cagtggacat
     7021 cgggaaggac ttggtaaagt tcaaagacca aatatgggga ctgctgatcg tgacaaagga
     7081 ctttgtttac tcccaaagtt ccaattgtct ttttgacaga aactacacac ttatgctaaa
     7141 agatcttttc ttgtctcgct tcaactcctt aatggtcttg ctctctcccc cagagccccg
     7201 atactcagat gacttgatat ctcaactatg ccagctgtac attgctgggg atcaagtctt
     7261 gtctatgtgt ggaaactccg gctatgaagt catcaaaata ttggagccat atgtcgtgaa
     7321 tagtttagtc cagagagcag aaaagtttag gcctctcatt cattccttgg gagactttcc
     7381 tgtatttata aaagacaagg taagtcaact tgaagagacg ttcggtccct gtgcaagaag
     7441 gttctttagg gctctggatc aattcgacaa catacatgac ttggtttttg tgtttggctg
     7501 ttacaggcat tgggggcacc catatataga ttatcgaaag ggtctgtcaa aactatatga
     7561 tcaggttcac cttaaaaaaa tgatagataa gtcctaccag gagtgcttag caagcgacct
     7621 agccaggagg atccttagat ggggttttga taagtactcc aagtggtatc tggattcaag
     7681 attcctagcc cgagaccacc ccttgactcc ttatatcaaa acccaaacat ggccacccaa
     7741 acatattgta gacttggtgg gggatacatg gcacaagctc ccgatcacgc agatctttga
     7801 gattcctgaa tcaatggatc cgtcagaaat attggatgac aaatcacatt ctttcaccag
     7861 aacgagacta gcttcttggc tgtcagaaaa ccgagggggg cctgttccta gcgaaaaagt
     7921 tattatcacg gccctgtcta agccgcctgt caatccccga gagtttctga ggtctataga
     7981 cctcggagga ttgccagatg aagacttgat aattggcctc aagccaaagg aacgggaatt
     8041 gaagattgaa ggtcgattct ttgctctaat gtcatggaat ctaagattgt attttgtcat
     8101 cactgaaaaa ctcttggcca actacatctt gccacttttt gacgcgctga ctatgacaga
     8161 caacctgaac aaggtgttta aaaagctgat cgacagggtc accgggcaag ggcttttgga
     8221 ctattcaagg gtcacatatg catttcacct ggactatgaa aagtggaaca accatcaaag
     8281 attagagtca acagaggatg tattttctgt cctagatcaa gtgtttggat tgaagagagt
     8341 gttttctaga acacacgagt tttttcaaaa ggcctggatc tattattcag acagatcaga
     8401 cctcatcggg ttacgggagg atcaaatata ctgcttagat gcgtccaacg gcccaacctg
     8461 ttggaatggc caggatggcg ggctagaagg cttacggcag aagggctgga gtctagtcag
     8521 cttattgatg atagatagag aatctcaaat caggaacaca agaaccaaaa tactagctca
     8581 aggagacaac caggttttat gtccgacata catgttgtcg ccagggctat ctcaagaggg
     8641 gctcctctat gaattggaga gaatatcaag gaatgcactt tcgatataca gagccgtcga
     8701 ggaaggggca tctaagctag ggctgatcat caagaaagaa gagaccatgt gtagttatga
     8761 cttcctcatc tatggaaaaa cccctttgtt tagaggtaac atattggtgc ctgagtccaa
     8821 aagatgggcc agagtctctt gcgtctctaa tgaccaaata gtcaacctcg ccaatataat
     8881 gtcgacagtg tccaccaatg cgctaacagt ggcacaacac tctcaatctt tgatcaaacc
     8941 gatgagggat tttctgctca tgtcagtaca ggcagtcttt cactacctgc tatttagccc
     9001 aatcttaaag ggaagagttt acaagattct gagcgctgaa ggggagagct ttctcctagc
     9061 catgtcaagg ataatctatc tagatccttc tttgggaggg atatctggaa tgtccctcgg
     9121 aagattccat atacgacagt tctcagaccc tgtctctgaa gggttatcct tctggagaga
     9181 gatctggtta agctcccaag agtcctggat tcacgcgttg tgtcaagagg ctggaaaccc
     9241 agatcttgga gagagaacac tcgagagctt cactcgcctt ctagaagatc cgaccacctt
     9301 aaatatcaga ggaggggcca gtcctaccat tctactcaag gatgcaatca gaaaggcttt
     9361 atatgacgag gtggacaagg tggaaaattc agagtttcga gaggcaatcc tgttgtccaa
     9421 gacccataga gataatttta tactcttctt aatatctgtt gagcctctgt ttcctcgatt
     9481 tctcagtgag ctattcagtt cgtctttttt gggaatcccc gagtcaatca ttggattgat
     9541 acaaaactcc cgaacgataa gaaggcagtt tagaaagagt ctctcaaaaa ctttagaaga
     9601 atccttctac aactcagaga tccacgggat tagtcggatg acccagacac ctcagagggt
     9661 tgggggggtg tggccttgct cttcagagag ggcagatcta cttagggaga tctcttgggg
     9721 aagaaaagtg gtaggcacga cagttcctca cccttctgag atgttgggat tacttcccaa
     9781 gtcctctatt tcttgcactt gtggagcaac aggaggaggc aatcctagag tttctgtatc
     9841 agtactcccg tcctttgatc agtcattttt ttcacgaggc cccctaaagg gatacttggg
     9901 ctcgtccacc tctatgtcga cccagctatt ccatgcatgg gaaaaagtca ctaatgttca
     9961 tgtggtgaag agagctctat cgttaaaaga atctataaac tggttcatta ctagagattc
    10021 caacttggct caagctctaa ttaggaacat tatgtctctg acaggccctg atttccctct
    10081 agaggaggcc cctgtcttca aaaggacggg gtcagccttg cataggttca agtctgccag
    10141 atacagcgaa ggagggtatt cttctgtctg cccgaacctc ctctctcata tttctgttag
    10201 tacagacacc atgtctgatt tgacccaaga cgggaagaac tacgatttca tgttccagcc
    10261 attgatgctt tatgcacaga catggacatc agagctggta cagagagaca caaggctaag
    10321 agactctacg tttcattggc acctccgatg caacaggtgt gtgagaccca ttgacgacgt
    10381 gaccctggag acctctcaga tcttcgagtt tccggatgtg tcgaaaagaa tatccagaat
    10441 ggtttctggg gctgtgcctc acttccagag gcttcccgat atccgtctga gaccaggaga
    10501 ttttgaatct ctaagcggta gagaaaagtc tcaccatatc ggatcagctc aggggctctt
    10561 atactcaatc ttagtggcaa ttcacgactc aggatacaat gatggaacca tcttccctgt
    10621 caacatatac ggcaaggttt cccctagaga ctatttgaga gggctcgcaa ggggagtatt
    10681 gataggatcc tcgatttgct tcttgacaag aatgacaaat atcaatatta atagacctct
    10741 tgaattggtc tcaggggtaa tctcatatat tctcctgagg ctagataacc atccctcctt
    10801 gtacataatg ctcagagaac cgtctcttag aggagagata ttttctatcc ctcagaaaat
    10861 ccccgccgct tatccaacca ctatgaaaga aggcaacaga tcaatcttgt gttatctcca
    10921 acatgtgcta cgctatgagc gagagataat cacggcgtct ccagagaatg actggctatg
    10981 gatcttttca gactttagaa gtgccaaaat gacgtaccta tccctcatta cttaccagtc
    11041 tcatcttcta ctccagaggg ttgagagaaa cctatctaag agtatgagag ataacctgcg
    11101 acaattgagt tctttgatga ggcaggtgct gggcgggcac ggagaagata ccttagagtc
    11161 agacgacaac attcaacgac tgctaaaaga ctctttacga aggacaagat gggtggatca
    11221 agaggtgcgc catgcagcta gaaccatgac tggagattac agccccaaca agaaggtgtc
    11281 ccgtaaggta ggatgttcag aatgggtctg ctctgctcaa caggttgcag tctctacctc
    11341 agcaaacccg gcccctgtct cggagcttga cataagggcc ctctctaaga ggttccagaa
    11401 ccctttgatc tcgggcttga gagtggttca gtgggcaacc ggtgctcatt ataagcttaa
    11461 gcctattcta gatgatctca atgttttccc atctctctgc cttgtagttg gggacgggtc
    11521 aggggggata tcaagggcag tcctcaacat gtttccagat gccaagcttg tgttcaacag
    11581 tcttttagag gtgaatgacc tgatggcttc cggaacacat ccactgcctc cttcagcaat
    11641 catgagggga ggaaatgata tcgtctccag agtgatagat cttgactcaa tctgggaaaa
    11701 accgtccgac ttgagaaact tggcaacctg gaaatacttc cagtcagtcc aaaagcaggt
    11761 caacatgtcc tatgacctca ttatttgcga tgcagaagtt actgacattg catctatcaa
    11821 ccggatcacc ctgttaatgt ccgattttgc attgtctata gatggaccac tctatttggt
    11881 cttcaaaact tatgggacta tgctagtaaa tccaaactac aaggctattc aacacctgtc
    11941 aagagcgttc ccctcggtca cagggtttat cacccaagta acttcgtctt tttcatctga
    12001 gctctacctc cgattctcca aacgagggaa gtttttcaga gatgctgagt acttgacctc
    12061 ttccaccctt cgagaaatga gccttgtgtt attcaattgt agcagcccca agagtgagat
    12121 gcagagagct cgttccttga actatcagga tcttgtgaga ggatttcctg aagaaatcat
    12181 atcaaatcct tacaatgaga tgatcataac tctgattgac agtgatgtag aatcttttct
    12241 agtccacaag atggttgatg atcttgagtt acagagggga actctgtcta aagtggctat
    12301 cattatagcc atcatgatag ttttctccaa cagagtcttc aacgtttcca aacccctaac
    12361 tgacccctcg ttctatccac cgtctgatcc caaaatcctg aggcacttca acatatgttg
    12421 cagtactatg atgtatctat ctactgcttt aggtgacgtc cctagcttcg caagacttca
    12481 cgacctgtat aacagaccta taacttatta cttcagaaag caagtcattc gagggaacgt
    12541 ttatctatct tggagttggt ccaacgacac ctcagtgttc aaaagggtag cctgtaattc
    12601 tagcctgagt ctgtcatctc actggatcag gttgatttac aagatagtga agactaccag
    12661 actcgttggc agcatcaagg atctatccag agaagtggaa agacaccttc ataggtacaa
    12721 caggtggatc accctagagg atatcagatc tagatcatcc ctactagact acagttgcct
    12781 gtgaaccgga tactcctgga agcctgccca tgctaagact cttgtgtgat gtatcttgaa
    12841 aaaaacaaga tcctaaatct gaacctttgg ttgtttgatt gtttttctca tttttgttgt
    12901 ttatttgtta agcgtgggtc ggcatggcat ctccacctcc tcgcggtccg acctgggcat
    12961 ccgaaggagg acgcacgtcc actcggatgg ctaagggagg ggcggggatc cggctgctaa
    13021 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
    13081 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
    13141 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
    13201 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    13261 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    13321 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    13381 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    13441 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    13501 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    13561 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    13621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    13681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    13741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    13801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    13861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    13921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    13981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    14041 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    14101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    14161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    14221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    14281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    14341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    14401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    14461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    14521 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    14581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    14641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    14701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    14761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    14821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    14881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    14941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    15001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    15061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    15121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    15181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    15241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    15301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    15361 catttccccg aaaagtgcca cctg
//
