LOCUS       sequence-81626-e        1142 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Cre recombinase expression plasmid for removal of floxed selectable 
            markers in the C. elegans germline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
  TITLE     Engineering the Caenorhabditis elegans genome using Cas9-triggered 
            homologous recombination.
  JOURNAL   Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
  PUBMED    23995389
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641."
COMMENT     Sequence Label: pDD104 (Peft-3::Cre)
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(108..128)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(140..164)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(173..772)
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    complement(773..793)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aacgcctggc gatccctgaa catgtccatc aggttcttgc gaacctcatc actcgttgca
       61 tcgaccggta atgcaggcaa attttggtgt acggtcagta aattggacac cttcctcttc
      121 ttcttgggtg cgcccatgga gcctgctttt ttgtacaaac ttgtgagcaa agtgtttccc
      181 aactgaaaaa aaaacaattt aatttaaaag taagaagagt gcggacggta gagagaataa
      241 aagtcgaaaa aattatccta gaaacttcta atatttttaa aaattaaaaa aatataaaaa
      301 gtgaggtcac aaataagaca gttagaacat ttgttgaaag cctaaaataa aaattcgact
      361 aaacagtgag aacaggaaaa acgctgggac tgaatgtttg acctcattat aaattcataa
      421 aatttaaatt tcctaatctg gataaaaaca aagataaaaa caaaataaac caacaaaaaa
      481 ccaacatgat tagtcagatg accagaaaac tcacgttaat ttcacaaggc ccaaaaaaat
      541 ctctccccct ctcgttgctg cctgcacatc taactcctag cacgaaaatg taccgtacac
      601 catttcaaac actctcggcg ggagtgttgc atactttttc tctctggcag tttattttca
      661 ataaattctt catttttata ttgtataatg tctttatttt atgaaaaatt cattaattta
      721 acagaaacaa tggaagaacc aatggaagtt gacaataaaa gaccaaaggt gccaactttt
      781 ctatacaaag ttgatagctt ggcgtaatca tggtcatagc tgttcctgcc taggtgagct
      841 ctggtaccct ctagtcaagg ccttaagtga gtcgtattac gtagcccggg cctagttcta
      901 gacattctct aatgaaaaaa tctttcagtt gaaattgaaa atgagttaaa gttggagttt
      961 ttattgaaaa cagatttccg tgtgattagt gtttttagcg agtgtgacag gacagcgaaa
     1021 aaatatagaa acaagggggg aactgaaaag cttaggaatg cattgaacat gagaagggga
     1081 aggggaagga acaaactaga caggaattat tggaatttaa tcacatttgg agtttttttt
     1141 ct
//
