LOCUS       sequence-81789-e         869 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  bacterial expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=300nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_300/pRSETB
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(283..360)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 gcagccggat caagcttcga attcttactg ctcgttcttc agcactctct ccacgaagct
       61 cttgatgtac ttgcccatct cgtcgggggc gtcctcctgg aggaagtgca ggcccttcac
      121 cttcacgaac tcggtgttgg ggaacttctt ggcgccctcc acgatggcgt tgctgaagaa
      181 gccggggtca ccctcgatga acagcttggg caggtcgtcg ctggctctca ggtaggcgtt
      241 gtagtttctc acgatctgca ccacgtcggg cttgccgagc tccagtgccc cggagctgga
      301 gatcttcttg aaccggttgg cagcgctgac ggcaatgaag tttttcttcc agcgcctctt
      361 ggacccgccc ccctttgctg tcatcatttg tacaaactct tcatagttta cttggccatc
      421 accatcgata tctgcttccc ttatcatttc atcaacttct tcatctgtta acttctcccc
      481 gaggtttgtc atgacgtgac gtaattgagc agcgctgatg tagccgttcc catccttgtc
      541 aaaaacacgg aatgcttctc ggatttcctc ttcgctgtct gtgtccttca tttttctagc
      601 catcatagta agaaattcag gaaagtaaat cgttccattg ccatcagcat cgacttcatt
      661 gatcatatcc tgcaattctg cttccgttgg gttttgtcca agcgacctca taacggtgcc
      721 aagttccttt gtggtgatgg tgccgtcccc atccttgtcg aataatgaga aggcttcttt
      781 gaactctgca atctgctctt ctgtcagttg gtcatgcatc catgggccct tcaccagggg
      841 gatctctctg ggccagctca gggtgggtc
//
