LOCUS       sequence-81817-e        1042 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses blasticidin for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, 
            Martin-Belmonte F, Mostov KE
  TITLE     A molecular network for de novo generation of the apical surface and
            lumen.
  JOURNAL   Nat Cell Biol. 2010 Oct 3. ():.
  PUBMED    20890297
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2010 Oct 3. ():."
COMMENT     Sequence Label: pLKO.1-blast
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             83..316
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(388..786)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(861..879)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ataaacaagt tcctctcact ctctgatatt catttctttg caagttataa atactgaata
       61 ataagatgac atgaactact actgctagag attttccaca ctgactaaaa gggtctgagg
      121 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
      181 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
      241 atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag
      301 tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc
      361 ttgtaagtca ttggtcttaa aggtacctta gccctcccac acataaccag agggcagcaa
      421 ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc ttcactatgg ctttgatccc
      481 aggatgcaga tcgagaagca cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt
      541 ctcatttccg atcgcgacga tacaagtcag gttgccagct gccgcagcag cagcagtgcc
      601 cagcaccacg agttctgcac aaggtccccc agtaaaatga tatacattga caccagtgaa
      661 gatgcggccg tcgctagaga gagctgcgct ggcgacgctg tagtcttcag agatggggat
      721 gctgttgatt gtagccgttg ctctttcaat gagggtggat tcttcttgag acaaaggctt
      781 ggccatggtg gatccccctg gggagagagg tcggtgattc ggtcaacgag ggagccgact
      841 gccgacgtgc gctccggagg cttgcagaat gcggaacacc gcgcgggcag gaacagggcc
      901 cacactaccg ccccacaccc cgcctcccgc accgcccctt cccggccgct gctctcggcg
      961 cgccctgctg agcagccgct attggccaca gcccatcgcg gtcggcgcgc tgccattgct
     1021 ccctggcgct gtccgtctgc ga
//
