LOCUS       sequence-81879-e         878 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Non-PIP3 binding control for PH-Btk-GFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Varnai P, Balla T
  TITLE     Visualization of phosphoinositides that bind pleckstrin homology 
            domains: calcium- and agonist-induced dynamic changes and 
            relationship to myo-[3H]inositol-labeled phosphoinositide pools.
  JOURNAL   J Cell Biol. 1998 Oct 19;143(2):501-10.
  PUBMED    9786958
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "1998-10-19"; volume: "143(2)"; pages: "501-10"
COMMENT     Sequence Label: PH-Akt(R25C)-GFP
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      646..655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(697..718)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attctgcatc
       61 agaggctgtg gccaggccag ctgggctcgg ggagcgccag cctgagagga gcgcgtgagc
      121 gtcgcgggag cctcgggcac catgagcgac gtggctattg tgaaggaggg ttggctgcac
      181 aaacgagggg agtacatcaa gacctggcgg ccatgctact tcctcctcaa gaatgatggc
      241 accttcattg gctacaagga gcggccgcag gatgtggacc aacgtgaggc tcccctcaac
      301 aacttctctg tggcgcagtg ccagctgatg aagacggagc ggccccggcc caacaccttc
      361 atcatccgct gcctgcagtg gaccactgtc atcgaacgca ccttccatgt ggagactcct
      421 gaggagcggg aggagtggac aaccgccatc cagactgtgg ctgacggcct caagaagcag
      481 gaggaggagg agatggactt ccggtcgggc tcacccagtg acaactcagg ggctgaagag
      541 atggaggtgt ccctggccaa gcccaagcac cgcgtgacca tgaacgagtt tgagtacctg
      601 aagctgctgg gcaagggcac tttcggcaag gtggatccac cggtcgccac catggtgagc
      661 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      721 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      781 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      841 accctgacct acggcgtgca gtgcttcagc cgctaccc
//
