LOCUS       sequence-82250-e         934 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses mCherry, nuclear mTagBFP and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-mCherry-N-P-M-nucBFP-EGFP-L
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      220..229
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             226..933
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(371..389)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     639..658
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctaga ttaagcgtct gatgagtccg tgaggacgaa
      121 acccggcgta ccgggtcacg cttaacaacc agatcaaaga aaaaacagac attgtcaatt
      181 gcaaagcaaa aatgtaacac ccctacaccg gtgaaagccg ccaccatggt gagcaagggc
      241 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      301 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      361 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      421 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      481 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      541 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      601 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      661 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      721 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      781 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      841 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      901 cactctaccg gcggcatgga cgagctgtac aagt
//
