LOCUS       sequence-82251-e         928 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses mCherry, nuclear mTagBFP and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-mCherry-N-P-M-nucBFP-EGFP-L
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             222..920
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 atcagattat atcccgcaaa tttatcactt gtttacctct ggaggagaga acatatgggc
       61 tcaactccaa cccttgggag caatataaca aagaacatgt tatggtgcca ttaaaccgct
      121 gcatttcatc aaagtcaagt tgattacctt tacattttga tcctcttgga tgtgaaaaaa
      181 actattaaca tccctcaaaa gaccccggga aagccgccac catgagcgag ctgattaagg
      241 agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca
      301 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
      361 agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca
      421 agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg
      481 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
      541 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca
      601 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc
      661 tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg
      721 ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc
      781 tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca
      841 acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca
      901 aactggggca caagcttaat tccggact
//
