LOCUS       sequence-82350-e         873 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of sgRNA precursor targeted against ade6+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ284
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(29..80)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     promoter        complement(185..541)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(578..594)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(578..594)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(591..613)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    602..618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(626..632,633..650,651..656))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    671..692
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(809..826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcgactcta gaggatcccc acggtacccc tgtttcgtcc tcacggactc atcagaccgg
       61 aaagcacatc cggtgacagg gcaccaccga ctcggtgcca ctcacttttt tcaagttgat
      121 aacggactag ccttatttta acttgctatt tctagctcta aaaccgaggc atctgaacaa
      181 tagattcttc ggtacaggtt atgttttttg gcaacagcaa ctattcaaat caactgaaag
      241 ttttttagaa ttcaacaact tccaatactc aagtgtccaa gctagcaaac tgtttaataa
      301 tcgtgaccca acattccttt catgcgcacc ctcagcgtca cataaatcaa cactttacaa
      361 cacctattca aagcttctat atgccttcgt catttaaatg tctaacattt caaaaaagtg
      421 tttgctaatg gtaaattctt gagttttctt ggaagcacat atataacgag tgtgactgtt
      481 ctacgctcct tgctgactag tcatcagtct acgcatgcca actaccacca acataagcaa
      541 aaggtacccg gggtaccgag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa
      601 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      661 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      721 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      781 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      841 ggctgcggcg agcggtatca gctcactcaa agg
//
