LOCUS       sequence-82493-e         819 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  bacterial expression of human HMG-CoA lyase (HL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Montgomery C, Miziorko HM
  TITLE     Influence of multiple cysteines on human 
            3-hydroxy-3-methylglutaryl-CoA lyase activity and formation of 
            inter-subunit adducts.
  JOURNAL   Arch Biochem Biophys. 2011 Jul;511(1-2):48-55. doi: 
            10.1016/j.abb.2011.04.004. Epub 2011 Apr 13.
  PUBMED    21514269
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Biochem Biophys."; date: "2011-07"; volume: "511(1-2):48-55. doi"; 
            pages: " 10.1016/j.abb.2011.04.004. Epub 2011 Apr 13"
COMMENT     Sequence Label: Human HMG-CoA lyase (HL)
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc ttgtcgacgg
       61 agctcgaatt cggatccagg tgggcaaggg gctcagagtt tacaggtagc ctgagccact
      121 ttggagctag tttttctgtt cagggcttga cagataaagt ttccagcttc cagaagcttc
      181 tggagattca cacccgtgtg aatgcccaag ccctctagca tgtagaccag gtcttctgtg
      241 gccaagtttc ctgatgcccc ctgtgcgtag ggacagcctc caagtcctgc cacagaagag
      301 tccacgacac tcactcccat ctgcagggcc atcaaggtgt tggtcagggc ttgaccatag
      361 gtgtcatggc agtggacagc cagggcagcc agaggcactt cctgcatgac agcagatagc
      421 atgtctttca tgatccctgg ggtgcccaca ccaatggtgt cccccaggga gatctcgtag
      481 cagcccattg agtagaactt cttggtgacc tcagctactt tagctgggga gatcttccct
      541 tcataagggc agccaagagc acaggagacg tacccccgca cagaaatatt ggctgactgc
      601 gctgccttca ggattgcgtc aaacctctga aaactctcct ctatggaaca attgatgttc
      661 ttcttggtga agagctctga ggcagctcca aagatgacta cttccttggc tccagcagca
      721 accgctgcct cgaagccttt caaatttggg gtcaggactg ggtagttgat gccaggaaac
      781 ttctgaatgc ccttcaagac ttcagtgtgg tcacccatc
//
