LOCUS       sequence-8256-ex       14149 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Luan H, Peabody NC, Vinson CR, White BH
  TITLE     Refined spatial manipulation of neuronal function by combinatorial 
            restriction of transgene expression.
  JOURNAL   Neuron. 2006 Nov 9. 52(3):425-36.
  PUBMED    17088209
REFERENCE   2  (bases 1 to 14149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Nov 9. 52(3):425-36."
COMMENT     Sequence Label: pCaST-elav-Gal4AD
FEATURES             Location/Qualifiers
     source          1..14149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     3015..3035
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3017..3035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3064..3080
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3065..3081
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6749..6769
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6785..7126
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     7132..7151
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7132..7150
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7157..7166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7169..7195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      8003..8012
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8009..8686
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(8189..8209)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8601..8624
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     intron          8778..8843
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8973..8993
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9265..9399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9302..9321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9356..9375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9426..9444)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(9904..9975,10370..10643,10718..11372,11434..11749,
                     11970..12101,12172..12786)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(13564..14118,14119..14149)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc
     3061 ccctcgaggt cgacggtatc gataagcttg atatcgaatt ccccggatcc agtcgaggga
     3121 tcactgaact aaaaagtaaa gagaggagaa acaggagaag cccttaaagt gcgagttcgc
     3181 tggagtgcct atcgatcctt gagggcagcg ctcgatcctt attactattg gcaggatcat
     3241 tttatacccc ttacataaaa atatcagtat cagtttgttc ccttatcatg cgtgctcttt
     3301 gacatatccg ggaatttatt tctatttttg cttagtcaaa ctgaagacca ttttgtccca
     3361 acataaaata tgtaaaatat ttttttttat cattttaaat tctcagggat taagtcaatg
     3421 tttgaaaact tctagctgac ttgcgctcca ctaccactcg gcatgcgtgc ccctgattag
     3481 taggccaggt ggtgggcctt tggcacgatc gcaaccctaa ggactgcaac cgaatagcat
     3541 tgagaaggag accaggattt ctgcactcaa aggtgaaatt gtctgagcac ggaacacgga
     3601 aaaacaacaa caaaaacatg gcagacggaa aacaatgccg ccagcggcaa ggagggaggg
     3661 tggaaacaca tgccacgatt cagtgtcgaa tgtcctgtga tcccacattc atatattttg
     3721 ttattgtgtg cctgcatccc tcgcctgctt ttaattcctg catcgatcct tctttgctcc
     3781 tggtctacga acccgatgtc ttatggtaat tttatgatta ttacaattag attcggggga
     3841 aactgcatct gccgccgtct gcagatccga ttcatttgga ttccgtctgc tcaatccaga
     3901 tccatgatgg gatcatggcc atttgggcat tggtacatgg aaagttaaaa gcgccctagg
     3961 cgttctaatc caactcgtct caagaaaaac gggtgaatgc atggaacgct ggatctggtt
     4021 ctggaagatc cggctaagtc aacaagaaat atgtaacctc gccattatag ctttactttc
     4081 gaagcagaaa atgcaatcga gaatatcgaa tgcacgctcc aggttcctat ggctcctagc
     4141 tttcggtgca gcacaacaaa tatttgggtt ggttcgtttt tccgccacga ttttaaaacg
     4201 ctaagggtgg ctttttcgga gtccctctca tttgagccgg gaaaaagacc agaccctccc
     4261 tttccctccc cagccacttt ccagctttgt tgtaacctcg tgttgtgcct gctttttaat
     4321 cccgctttgt tgtaacgcag gccatttgct gtttttgttt tgtttctggc gtggctaaaa
     4381 cggattatgt gacccaaccc tttcgatttc gagtctcatt gtattacatc cttttcccaa
     4441 aaatgactaa aaaagcgtat tgcctatttc tggggtggac agaatgaaac ggggtcagca
     4501 cgtggccgca aacttgaaat accattatgg atccaatagg actgtgaaga gttattagtg
     4561 aaacatttcg aacaaactat tcctttaatt gaaatttgcc aattccccac ttaagcatcg
     4621 catttgcata tttgtatgct aaccaaactc tctgcgacgt cgatctgtct gaatctgaat
     4681 ccgcatatct ttcgagggca gcaatagcgc caccccaccc catttccgtt gccactagca
     4741 gcgcaccacc ctttgaaagg acatcaaaaa ccaccccctg cccaaagttg cgttacaatt
     4801 cccgaagtgc agtgcagcgt acataaaaca tacaatgaaa tccgataaaa ttttctatgt
     4861 cctttatatc ctcataacat gcccaactat aaaccgagcc aaattcttct cgtgaaaaag
     4921 ttgcacacaa cgcgatttca ctgtacctta cattgttctg taaactggaa cagagcgtcc
     4981 gactggaaga ggcgaaggaa aaaatcagca cagggaacag gaagttcaca aggattgggg
     5041 gtgtccgagg gtggctacat atatatggcg atatatggca tacagtatgt gcatacatat
     5101 acatacatat acacatatat gtatgtatgt accatactat gtggcgtgtc tagtggcgcg
     5161 aataacgaat acaggagcaa tgaagcacac gaacgaagcg aaacgaaacg aagggaaata
     5221 ttgaaaaagc cagaattgca ttacttcaaa actataccgg ctcttggata tgggtcctgg
     5281 gtctcggttc cttgcttctg gctttcgctt cgtagggggt gtacgagcaa tactgccagg
     5341 agaaaccacc ctagcctttg gcgttggtgg ttgatagtgc aaaaataata ataacggaaa
     5401 cggaaccata aacccaaaac agcccttaca acgcgactga tttgtaagga taatggtcgc
     5461 atgcatcata aaacggttga cgacctcgtc ggaaagagcc aacattttgc aatgatttcc
     5521 aattgctcct gctatctagg cccaatgggc cgctaagtac atatgaagca tacatagcta
     5581 aatacatatg aagcatacat acatcgggtt aactataaaa ccaatgtgtt ctagatgagt
     5641 ttgttaattt tacaaagtaa gttagggctg ctccagtggt tttaagttgc ttaactggac
     5701 aagagaactt ggctctctcc cccatctatc tcttgtcttc gggaaaggta acctccattt
     5761 tgatcggttg aatttgtatc aaaaagcatg aaaaactttg aatacagcat aaaccactga
     5821 gagagcagcc gaaagccagg ggtgtatcaa aaggggttct tagaaatatt ccactttgca
     5881 acgattatag actcgtttct tatcagacaa aactttctcc acttctacgt ggtatttaca
     5941 gaaacaaaac ttaaggtaaa atgttaggtg gctatatatt aaagctatta aaatatatta
     6001 aaggtttccg gaataacccc ctatctcggc gcgcagatac gcccatgtcg ccactctttc
     6061 gctctctacc ttccactctc gctccgaaca ccattgcaac cccgagagag ctgctcgaga
     6121 ggcaactatg agatatgaga atgagaggtt gctctatgtg aaaatggaaa ccgaaaccgt
     6181 caatgcctca atttcgcatt tcattatttc atttccacgc caaccatcgt gcgccgcggt
     6241 ctgaacgctc ctgccacaga aaaagaagat aaaagcaagg aaaattctaa tcgaatacca
     6301 aatatcgtgc ttgtgtgctc tttccgcaat tgattttttt taagtagtgc atgacaataa
     6361 ccgttgagtt gactccaacc gaagtaacca taactggaag ctgacaacag ccattggccg
     6421 gaatctcatc tcacaccgag cgaaatacgg cgacgccagc gtttatttat taaaaacgat
     6481 tacattacaa caaacaccaa agtcctggac aaccgaagag agtgtgggag aaaatcgaag
     6541 cagagagcaa ggaggagcgc cgtgagagcg cgcgcgcaag gatttgagat tgcaaagaga
     6601 acttagcaat tagttcgctt tgtttgtcca gccaaacaac aacaacaacc aaccagctcc
     6661 ccaaaaccaa ctacacacat actcgctccc taaactaaaa caatagcggc cgcaaaacat
     6721 ggataaagcg gaattaattc ccgagcctcc aaaaaagaag agaaaggtcg aattgggtac
     6781 cgccgccaat tttaatcaaa gtgggaatat tgctgatagc tcattgtcct tcactttcac
     6841 taacagtagc aacggtccga acctcataac aactcaaaca aattctcaag cgctttcaca
     6901 accaattgcc tcctctaacg ttcatgataa cttcatgaat aatgaaatca cggctagtaa
     6961 aattgatgat ggtaataatt caaaaccact gtcacctggt tggacggacc aaactgcgta
     7021 taacgcgttt ggaatcacta cagggatgtt taataccact acaatggatg atgtatataa
     7081 ctatctattc gatgatgaag ataccccacc aaacccaaaa aaagagatct ttaatacgac
     7141 tcactatagg gcgagcgccg ccatggagta cccatacgac gtaccagatt acgctggagg
     7201 aggtggtgga ggtggaggtg gaggtactag tctggagatc gaggccgcct tcctggagcg
     7261 cgagaacacc gccctggaga cccgcgtggc cgagctgcgc cagcgcgtgc agcgcctgcg
     7321 caaccgcgtg agccagaacc gcacccgcta cggccccctg ggcggcggca agtaaggcgc
     7381 gcctctagga tccaaatgca attggctaaa aaccgaaacc aaaacgcaac aaagtatacg
     7441 aaacacttgt gaaaccgtac aaacaattgt ggaaaaaaaa ttaaaagatt attaagattg
     7501 aagtctcaat aaacattagt gcttaaataa acttaaagcg acccgcgtgg agagtgcagt
     7561 aaaaagaata acttttgaaa taaatattta ccaaacagaa aaatatttta taaatattta
     7621 aataagtgaa aaacaaattg gttactctga aacaaagaat attcaaattg gcgctaaaac
     7681 aaaggagaaa aaatttcaag aatattatac agataataag acatatttaa ctatataaaa
     7741 ccaaacttaa tcaacaaaga caaaggagtg aaaaaaataa aaaaaaattt taaaagagtt
     7801 aaaaaaaaat tgtttatatc caaaggaggc aaaggaacag cacagaaagc gaggaaacac
     7861 tcaaataaaa tccgccaaaa atcgcagatc cctggaaacc aattcgtgtg aaatcggtca
     7921 agcccccaac gacttttagc ccgtcccaga cggagcaccg ccaagattct taccgccagc
     7981 agcgccatga tcaagctcgg tcgccaccat ggcctcctcc gagaacgtca tcaccgagtt
     8041 catgcgcttc aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg
     8101 cgagggcgag ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg
     8161 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt
     8221 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt
     8281 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gcgaccgtga cccaggactc
     8341 ctccctgcag gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc
     8401 cgacggcccc gtgatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     8461 cccccgcgac ggcgtgctga agggcgagac ccacaaggcc ctgaagctga aggacggcgg
     8521 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg
     8581 ctactactac gtggacgcca agctggacat cacctcccac aacgaggact acaccatcgt
     8641 ggagcagtac gagcgcaccg agggccgcca ccacctgttc ctgtagcggc ctcgactaga
     8701 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     8761 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     8821 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     8881 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     8941 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     9001 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     9061 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     9121 ttatggaaaa atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     9181 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     9241 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     9301 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9361 ttgtccaaac tcatcaatgt atcttatcat gtctggatcg gatccactag aaggccttag
     9421 tatgtatgta agttaataaa accctttttt ggagaatgta gatttaaaaa aacatatttt
     9481 ttttttattt tttactgcac tggacatcat tgaacttatc tgatcagttt taaatttact
     9541 tcgatccaag ggtatttgaa gtaccaggtt ctttcgatta cctctcactc aaaatgacat
     9601 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
     9661 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacctag cgtcaatgtc
     9721 cgccttcagt tgcactttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
     9781 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
     9841 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
     9901 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
     9961 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
    10021 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
    10081 aaaccgcaat cgcacaaaca aatagtgaca cgaaacagat tattctggta gctgtgctcg
    10141 ctatataaga caatttttaa gatcatatca tgatcaagac atctaaaggc attcattttc
    10201 gactacattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
    10261 acaaaaaata aataaaagta taaacctact tcgtaggata cttcgttttg ttcggggtta
    10321 gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg
    10381 agcggcttcg cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct
    10441 cctgccaccc agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct
    10501 cacctatgcc tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg
    10561 gcggcagctg gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc
    10621 caggaaacat ttgctcaaga acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa
    10681 tcttaataaa gggtccaatt accaatttga aactcagttt gcggcgtggc ctatccgggc
    10741 gaacttttgg ccgtgatggg cagttccggt gccggaaaga cgaccctgct gaatgccctt
    10801 gcctttcgat cgccgcaggg catccaagta tcgccatccg ggatgcgact gctcaatggc
    10861 caacctgtgg acgccaagga gatgcaggcc aggtgcgcct atgtccagca ggatgacctc
    10921 tttatcggct ccctaacggc cagggaacac ctgattttcc aggccatggt gcggatgcca
    10981 cgacatctga cctatcggca gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg
    11041 ctcagcaaat gtcagcacac gatcatcggt gtgcccggca gggtgaaagg tctgtccggc
    11101 ggagaaagga agcgtctggc attcgcctcc gaggcactaa ccgatccgcc gcttctgatc
    11161 tgcgatgagc ccacctccgg actggactca tttaccgccc acagcgtcgt ccaggtgctg
    11221 aagaagctgt cgcagaaggg caagaccgtc atcctgacca ttcatcagcc gtcttccgag
    11281 ctgtttgagc tctttgacaa gatccttctg atggccgagg gcagggtagc tttcttgggc
    11341 actcccagcg aagccgtcga cttcttttcc tagtgagttc gatgtgttta ttaagggtat
    11401 ctagcattac attacatctc aactcctatc cagcgtgggt gcccagtgtc ctaccaacta
    11461 caatccggcg gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc
    11521 ccgtgatcgg atcgccaaga tatgcgacaa ttttgctatt agcaaagtag cccgggatat
    11581 ggagcagttg ttggccacca aaaatttgga gaagccactg gagcagccgg agaatgggta
    11641 cacctacaag gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc
    11701 ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc
    11761 cagtggaaac aaatgatata acgcttacaa ttcttggaaa caaattcgct agattttagt
    11821 tagaattgcc tgattccaca cccttcttag tttttttcaa tgagatgtat agtttatagt
    11881 tttgcagaaa ataaataaat ttcatttaac tcgcgaacat gttgaagata tgaatattaa
    11941 tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt
    12001 tgggccaaca actcacgcaa gtgggcgtga tgaatatcaa cggagccatc ttcctcttcc
    12061 tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtctt gtttagaata
    12121 catttgcata ttaataattt actaactttc taatgaatcg attcgattta ggtgttcacc
    12181 tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac
    12241 tttctgggca aaacgattgc cgaattaccg ctttttctca cagtgccact ggtcttcacg
    12301 gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg
    12361 gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc
    12421 agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc
    12481 tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
    12541 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
    12601 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
    12661 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt
    12721 ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag
    12781 gagtagccga catatatccg aaataactgc ttgttttttt ttttaccatt attaccatcg
    12841 tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac
    12901 aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg
    12961 gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta
    13021 tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct
    13081 cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc
    13141 gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg
    13201 cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta
    13261 ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag
    13321 ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg
    13381 agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat
    13441 gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta
    13501 taataataag aatacattta atttagaaaa tgcttggatt tcactggaac tagaattaat
    13561 tcggctgctg ctctaaacga cgcatttcgt actccaaagt acgaattttt tccctcaagc
    13621 tcttattttc attaaacaat gaacaggacc taacgcacag tcacgttatt gtttacataa
    13681 atgatttttt ttactattca aacttactct gtttgtgtac tcccactggt atagccttct
    13741 tttatctttt ctggttcagg ctctatcact ttactaggta cggcatctgc gttgagtcgc
    13801 ctccttttaa atgtctgacc ttttgcaggt gcagccttcc actgcgaatc attaaagtgg
    13861 gtatcacaaa tttgggagtt ttcaccaagg ctgcacccaa ggctctgctc ccacaatttt
    13921 ctcttaatag cacacttcgg cacgtgaatt aattttactc cagtcacagc tttgcagcaa
    13981 aatttgcaat atttcatttt tttttattcc acgtaagggt taatgttttc aaaaaaaaat
    14041 tcgtccgcac acaacctttc ctctcaacaa gcaaacgtgc actgaattta agtgtatact
    14101 tcggtaagct tcggctatcg acgggaccac cttatgttat ttcatcatg
//
