LOCUS       sequence-82620-e         826 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  bacterial expression of human SPOP (aa 28-337) fused to His-MBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, 
            Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, 
            White KP, Schulman BA
  TITLE     Structures of SPOP-substrate complexes: insights into molecular 
            architectures of BTB-Cul3 ubiquitin ligases.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
  PUBMED    19818708
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "39-50"
COMMENT     Sequence Label: His-MBP human SPOP (aa 28-337)
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     97..116
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        97..115
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    116..140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             196..213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgc ccgggatctc
       61 gacgctctcc cttatgcgac tcctgcatta ggaaattaat acgactcact ataggggaat
      121 tgtgagcgga taacaattcc cctgtagaaa taattttgtt taactttaat aaggagatat
      181 accatgggtt cttctcacca tcaccatcac cacggttctt ctatgaaaat cgaagaaggt
      241 aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
      301 aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
      361 aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
      421 cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
      481 caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
      541 tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
      601 ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
      661 gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
      721 ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
      781 gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaa
//
