LOCUS       sequence-82632-e         890 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral expression vector for TAZ S89A mutant with N-terminal 
            FLAG and His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, 
            Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, 
            Kitagawa M, Hata Y
  TITLE     Screening with a novel cell-based assay for TAZ activators 
            identifies a compound that enhances myogenesis in C2C12 cells and 
            facilitates muscle repair in the muscle injury model.
  JOURNAL   Mol Cell Biol. 2014 Feb 18.
  PUBMED    24550007
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2014 Feb 18."
COMMENT     Sequence Label: pLenti-EF-FH-TAZ S89A-ires-blast
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..57
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             64..81
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcttgttaa cgtttaaact agccggggcc atggactaca aagacgatga cgacaaggaa
       61 ttgcaccatc atcatcatca tgaattcaat ccggcctcgg cgccccctcc gctcccgccg
      121 cctgggcagc aagtgatcca cgtcacgcag gacctagaca cagacctcga agccctcttc
      181 aactctgtca tgaatccgaa gcctagctcg tggcggaaga agatcctgcc ggagtctttc
      241 tttaaggagc ctgattcggg ctcgcactcg cgccagtcca gcaccgactc gtcgggcggc
      301 cacccggggc ctcgactggc tgggggtgcc cagcatgtcc gctcgcatgc gtcgcccgcg
      361 tccctgcagc tgggcaccgg cgcgggtgct gcgggtagcc ccgcgcagca gcacgcgcac
      421 ctccgccagc agtcctacga cgtgaccgac gagctgccac tgcccccggg ctgggagatg
      481 accttcacgg ccactggcca gaggtacttc ctcaatcaca tagaaaaaat caccacatgg
      541 caagacccta ggaaggcgat gaatcagcct ctgaatcata tgaacctcca ccctgccgtc
      601 agttccacac cagtgcctca gaggtccatg gcagtatccc agccaaatct cgtgatgaat
      661 caccaacacc agcagcagat ggcccccagt accctgagcc agcagaacca ccccactcag
      721 aacccacccg cagggctcat gagtatgccc aatgcgctga ccactcagca gcagcagcag
      781 cagaaactgc ggcttcagag aatccagatg gagagagaaa ggattcgaat gcgccaagag
      841 gagctcatga ggcaggaagc tgccctctgt cgacagctcc ccatggaagc
//
