LOCUS       sequence-82642-e         888 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Please note this plasmid is discontinued. If you need this plasmid 
            for experiments, please directly contact Robert Campbell at 
            University of Alberta..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Belal AS, Sell BR, Hoi H, Davidson MW, Campbell RE
  TITLE     Optimization of a genetically encoded biosensor for cyclin B1-cyclin
            dependent kinase 1.
  JOURNAL   Mol Biosyst. 2014 Feb;10(2):191-5. doi: 10.1039/c3mb70402e.
  PUBMED    24281384
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-02"; volume: "10"; issue: "2"; pages: 
            "191-5"
COMMENT     Sequence Label: cyclin B1-CDK1 FRET biosensor
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..808
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 agctctctgg ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctag ccgccaccat ggcctcgagc atggtgagca agggcgagga
      121 gaccacaatg ggcgtaatca agcccgacat gaagatcaag ctgaagatgg agggcaacgt
      181 gaatggccac gccttcgtga tcgagggcga gggcgagggc aagccctacg acggcaccaa
      241 caccatcaac ctggaggtga aggagggagc ccccctgccc ttctcctacg acattctgac
      301 caccgcgttc gcctacggca acagggcctt caccaagtac cccgacgaca tccccaacta
      361 cttcaagcag tccttccccg agggctactc ttgggagcgc accatgacct tcgaggacaa
      421 gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca tctacgagat
      481 acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaaga agaccaccgg
      541 ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg gcgacgtcaa
      601 gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga ccatctacag
      661 ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca tcgagatcct
      721 gaaccacgac aaggactaca acaaggtgac cgtttacgag agcgccgtgg cccgcaactc
      781 caccgacggc atggacgagc tgtacaagcc taggttaact ttaaaaccat tgcctgacag
      841 cattattcaa gaaagcctgg agattcagca aggtgtgaat ccattttt
//
