LOCUS       sequence-82736-e         967 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-EF05
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(120..563)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKNADGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     679..698
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     938..959
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca
       61 ccacagaagt aaggttcctt cacaaagatc ctctagaggt accctcgagc cgcggccgct
      121 cacttcgctg tcatcatttg tacaaactct tcgtagttta cctgaccatc cccatcgatg
      181 tctgcttccc tgatcatttc atcaacctct tcatctgtta acttctctcc aaggtttgtc
      241 atcacgtggc gaagctctgc tgcactgatg tagccatcgg cattcttatc aaacacacgg
      301 aacgcttctc taatttcttc ttcactgtct gtgtctttca tttttcttgc catcattgtc
      361 aggaactcag ggaagtcgat tgtgccgtca ccgtcggcat ctacttcatt gatcatgtcc
      421 tgcagctctg cttctgtggg gttctgcccc agagaccgca tcaccgtccc cagctccttg
      481 gttgttattg tcccatcccc gtccttgtca aatagggaga aagcctcttt aaattctgcg
      541 atctgctctt cagtcagttg gtcacgcgtg ttgtactcca gcttgtgccc caggatgttg
      601 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      661 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
      721 tggatgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
      781 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
      841 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      901 tcaccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      961 atgggca
//
