LOCUS       sequence-82972-e        9676 bp    DNA     circular SYN 15-APR-2025
DEFINITION  GAL driven LexA-tagged SET1-catalytically dead (H1017K).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Rizzardi LF, Dorn ES, Strahl BD, Cook JG
  TITLE     DNA replication origin function is promoted by H3K4 di-methylation 
            in Saccharomyces cerevisiae.
  JOURNAL   Genetics. 2012 Oct;192(2):371-84. doi: 10.1534/genetics.112.142349. 
            Epub 2012 Jul 30.
  PUBMED    22851644
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.112.142349"; journalName: "Genetics"; date: 
            "2012-10"; volume: "192"; issue: "2"; pages: "371-84"
COMMENT     Sequence Label: pGLx2-set1-H1017K
FEATURES             Location/Qualifiers
     source          1..9676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3255..3272)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3255..3271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3264..3286)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(3544..4347)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4348..4558)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      4794..5958
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     complement(5975..5994)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6094..6116
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6154..6172)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6240..6344
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6345..6413,6414..7205)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6563..6582)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7376..7964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7865..7884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8118..8135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(8288..8293,8294..8311,8312..8318)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8326..8342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8331..8353
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8350..8366
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8350..8366
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8590..9031
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    8590..8707
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     9002..9025
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             join(9055..9306,9307..9660)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     9598..9619
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 gggactcaaa ttactataga agagcacacg cgtcttctgg ttcatacaga caaccccagg
       61 aacagcctca atattcgcgt tctggtcact atcagtattc aaacggccat tctcaccaac
      121 aatattctag tcaatataat caacgtcgac gttataacca taatgatggt acaaggcgac
      181 gctataatga cgatcgccca catagttcaa acaatgcaag tacgcgacag tactatgcta
      241 ctaacaacag ccaaagcggc ccatatgtaa ataagaaatc tgacatcagt agtcggaggg
      301 gcatgtctca atcacggtat tcaaatagca atgttcacaa tacattagcg tcttcgagtg
      361 gatctcttcc cacagaatct gctctgcttt tgcaacaaag accaccttca gttttgagat
      421 acaacacaga taatttgaag tctaagtttc attattttga tcccataaaa ggcgagttct
      481 tcaataagga taagatgctt tcgtggaagg ctacagataa agaattttct gaaacaggtt
      541 attacgtagt caaagagtta caagatggac agtttaagtt caaaataaaa cacagacatc
      601 cggagataaa agcatccgac ccacgtaatg aaaacggtat catgactagc ggaaaagtgg
      661 caacccacag aaaatgcagg aactcactaa ttctattgcc tcgcatatct tatgacaggt
      721 actccttagg gcctccccct tcatgtgaaa tagttgtcta tccagcgcaa gattcaacaa
      781 caaccaatat ccaagacata tcaataaaaa actattttaa aaagtatgga gaaatttctc
      841 attttgaagc atttaatgat cctaatagcg ctttaccttt gcatgtttat cttataaagt
      901 atgccagttc tgatggaaaa attaatgatg cagcaaaagc agcctttagt gccgttagaa
      961 agcacgaatc ttcgggttgc tttatcatgg gcttcaagtt cgaagtgatt ttaaacaagc
     1021 attccatttt gaataatatc atttctaaat ttgttgaaat aaatgtcaaa aagctacaga
     1081 agttacaaga gaacctgaag aaggctaaag agaaagaagc agaaaacgaa aaagcaaagg
     1141 aattacaggg caaagatatt accttgccca aggaacctaa ggtagacaca ttatctcatt
     1201 cgtccggaag tgaaaaaaga attccatatg atctcttggg ggtagttaat aacagacctg
     1261 ttttacatgt ctccaaaata tttgttgcca aacataggtt ctgcgttgag gactttaaat
     1321 acaagttaag gggatacaga tgtgcgaaat ttattgatca tccaactggt atctatatta
     1381 tttttaatga cattgcccat gcgcaaacat gttcgaatgc agagtcagga aatttaacaa
     1441 taatgtctcg gagcagaaga attcctattc taataaagtt tcatctcatt ctccctaggt
     1501 tccaaaacag aactagattc aataaatcta gctcatcttc aaattctaca aatgtaccta
     1561 taaaatacga gtccaaagag gagttcattg aagctacagc aaaacaaata ttaaaagatt
     1621 tggaaaagac tttacatgtt gatattaaga agagattgat tggtcctacg gtatttgatg
     1681 ctttggacca tgcaaatttt cctgaattgt tagctaaaag agaactaaag gagaaagaga
     1741 agagacaaca gattgcatct aaaattgctg aagatgaatt gaaacgtaaa gaagaagcca
     1801 aaagagattt tgatttgttt ggtttatatg gtggctatgc aaaatctaat aaaagaaatt
     1861 taaaaaggca taattcactc gcgttggatc atacttcttt aaagaggaaa aagctatcca
     1921 atggtatcaa accaatggca catttactga acgaagaaac cgattccaaa gaaactaccc
     1981 cattgaacga tgaagggatc actcgcgtat caaaagaaca tgatgaagaa gacgaaaata
     2041 tgacatcttc atcttctgaa gaagaggaag aagaagctcc agataagaaa ttcaagagtg
     2101 agtctgagcc aaccaccccc gaatctgatc accttcatgg tattaagccg ttagtacccg
     2161 atcaaaatgg gtcgtctgac gtactggatg cttcttcgat gtataaacct actgctaccg
     2221 aaattcccga acctgtatat ccacctgagg aatatgactt gaaatatagt cagactttat
     2281 cttctatgga tttgcagaat gctatcaaag atgaggaaga tatgctaatt ttaaagcagt
     2341 tattgagcac atatactcct accgtcacac cagaaacaag cgcagctctg gaatataaaa
     2401 tttggcaatc tcgccgaaaa gttcttgaag aagagaaggc ttccgattgg caaatagagc
     2461 ttaatggaac tttatttgat agtgaactac aaccaggtag ctcttttaaa gctgaagggt
     2521 tcaggaaaat tgcggataaa ttaaaaatta attacctacc ccatcgtcgc agagttcacc
     2581 aacctttaaa tacggtgaat attcacaatg aaaggaatga gtacacacct gaactttgtc
     2641 aaagagaaga atcctcgaat aaagaacctt cagactcagt tcctcaagaa gtttcatcct
     2701 ctagagataa tagggcatca aatagaagat ttcagcagga catagaggca cagaaagccg
     2761 caattggtac ggaatctgag ctgctatcac taaatcaatt aaataaaaga aaaaagccag
     2821 ttatgttcgc tcgttcagca attcacaact ggggtttata tgctctagac tctatcgcag
     2881 caaaggaaat gattatcgag tacgttggtg aaaggatcag gcaacctgta gcagaaatga
     2941 gagagaaaag atatctgaaa aatgggattg gatccagtta cctttttagg gttgatgaaa
     3001 acacggttat tgatgccacc aagaaaggtg gtatagcccg tttcattaat aagtgttgtg
     3061 atccaaattg tacggcaaag attataaagg ttggcgggag aaggagaatt gttatctatg
     3121 cactgcgtga tatcgcggca agcgaagagt tgacatatga ttacaaattt gagagagaaa
     3181 aggatgacga ggaaagactt ccttgtttat gtggagcacc taattgtaaa ggtttcttga
     3241 actgaaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3301 acccaactta atcgccttgc agcacatccc cccttcgcca gctggcgtaa tagcgaagag
     3361 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     3421 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggta ataactgata
     3481 taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt
     3541 tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg
     3601 ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat
     3661 gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc
     3721 cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc
     3781 acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc
     3841 aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa
     3901 catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac
     3961 aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta
     4021 tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc
     4081 gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat
     4141 ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc
     4201 ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt
     4261 ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc
     4321 acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcggttttt gttctgtgca
     4381 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata
     4441 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
     4501 aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa
     4561 gctcttgtta cccatcattg aattttgaac atccgaacct gggagttttc cctgaaacag
     4621 atagtatatt tgaacctgta taataatata tagtctagcg ctttacggaa gacaatgtat
     4681 gtatttcggt tcctggagaa actattgcat ctattgcata ggtaatcttg cacgtcgcat
     4741 ccccggttca ttttctgcgt ttccatcttg cacttcaata gcatatcttt gttaacgaag
     4801 catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
     4861 agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac
     4921 gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca
     4981 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta
     5041 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt
     5101 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc
     5161 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat
     5221 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc
     5281 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt
     5341 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta
     5401 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt
     5461 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat
     5521 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa
     5581 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt
     5641 gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg
     5701 tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag
     5761 ttcctatact ttctagctag agaataggaa cttcggaata ggaacttcaa agcgtttccg
     5821 aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc
     5881 gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt
     5941 gtttatgctt aaatgcgtta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6001 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6061 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6121 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     6181 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6241 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6301 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6361 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6421 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6481 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6541 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6601 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6661 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6721 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6781 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6841 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6901 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6961 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7021 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7081 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7141 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7201 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7261 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7321 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7381 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7441 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7501 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     7561 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7621 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7681 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7741 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     7801 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7861 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7921 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7981 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8041 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8101 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8161 aaccgcctct ccccgcgcgt tggccgattc attaatccag ctggcacgac aggtttcccg
     8221 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact cattaggcac
     8281 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8341 aatttcacac aggaaacagc tatgaccatg attacgaatt cgaattttca aaaattctta
     8401 cttttttttt ggatggacgc aaagaagttt aataatcata ttacatggca ttaccaccat
     8461 atacatatcc atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct
     8521 tagcctaaaa aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat
     8581 attgaagtac ggattagaag ccgccgagcg ggcgacagcc ctccgacgga agactctcct
     8641 ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac
     8701 tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg
     8761 gcagtaacct ggccccacaa accttcaaat taacgaatca aattaacaac cataggatga
     8821 taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt
     8881 tgatctatta acagatatat aaatggaaaa gctgcataac cactttaact aatactttca
     8941 acattttcag tttgtattac ttcttattca aatgtcataa aagtatcaac aaaaaattgt
     9001 taatatacct ctatacttta acgtcaagga gaaaaaacgg atcattcggc cgccatgaaa
     9061 gcgttaacgg ccaggcaaca agaggtgttt gatctcatcc gtgatcacat cagccagaca
     9121 ggtatgccgc cgacgcgtgc ggaaatcgcg cagcgtttgg ggttccgttc cccaaacgcg
     9181 gctgaagaac atctgaaggc gctggcacgc aaaggcgtta ttgaaattgt ttccggcgca
     9241 tcacgcggga ttcgtctgtt gcaggaagag gaagaagggt tgccgctggt aggtcgtgtg
     9301 gctgccggtg aaccacttct ggcgcaacag catattgaag gtcattatca ggtcgatcct
     9361 tccttattca agccgaatgc tgatttcctg ctgcgcgtca gcgggatgtc gatgaaagat
     9421 atcggcatta tggatggtga cttgctggca gtgcataaaa ctcaggatgt acgtaacggt
     9481 caggtcgttg tcgcacgtat tgatgacgaa gttaccgtta agcgcctgaa aaaacagggc
     9541 aataaagtcg aactgttgcc agaaaatagc gagtttaaac caattgtcgt agatcttcgt
     9601 cagcagagct tcaccattga agggctggcg gttggggtta ttcgcaacgg cgactggctg
     9661 ggatcctcga gcgccc
//
