LOCUS       sequence-82990-e        9476 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway 
            gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and 
            thereby produces lycopene in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Cunningham FX Jr, Sun Z, Chamovitz D, Hirschberg J, Gantt E
  TITLE     Molecular structure and enzymatic function of lycopene cyclase from 
            the cyanobacterium Synechococcus sp strain PCC7942.
  JOURNAL   Plant Cell. 1994 Aug;6(8):1107-21.
  PUBMED    7919981
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Cell."; date: "1994-08"; volume: "6(8)"; pages: "1107-21"
COMMENT     Sequence Label: pAC-LYC
FEATURES             Location/Qualifiers
     source          1..9476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1505..1510,1511..1527,1528..1533)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(7164..7183)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(9036..9476,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
     1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
     1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
     2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
     2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
     2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
     2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
     2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
     2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
     2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
     2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
     2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
     2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
     2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
     2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
     2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
     2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
     2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
     2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
     3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
     3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
     3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
     3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
     3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
     3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
     3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
     3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
     3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
     3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
     3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
     3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
     3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
     3781 cccttattgc ctcgggaatg cgggctgggc tatttcaccc taccactggc tattcgctgc
     3841 cgctggcggt ggcccttgcc gacgcgattg ccgacagccc gcggctgggc agcgttccgc
     3901 tctatcagct cacccggcag tttgccgaac gccactggcg caggcaggga ttcttccgcc
     3961 tgctgaaccg gatgcttttc ctggccgggc gcgaggagaa ccgctggcgg gtgatgcagc
     4021 gcttttatgg gctgccggag cccaccgtag agcgctttta cgccggtcgg ctctctctct
     4081 ttgataaggc ccgcattttg acgggcaagc caccggttcc gctgggcgaa gcctggcggg
     4141 cggcgctgaa ccattttcct gacagacgag ataaaggatg aaaaaaaccg ttgtgattgg
     4201 cgcaggcttt ggtggcctgg cgctggcgat tcgcctgcag gcggcaggga tcccaaccgt
     4261 actgctggag cagcgggaca agcccggcgg tcgggcctac gtctggcatg accagggctt
     4321 tacctttgac gccgggccga cggtgatcac cgatcctacc gcgcttgagg cgctgttcac
     4381 cctggccggc aggcgcatgg aggattacgt caggctgctg ccggtaaaac ccttctaccg
     4441 actctgctgg gagtccggga agaccctcga ctatgctaac gacagcgccg agcttgaggc
     4501 gcagattacc cagttcaacc cccgcgacgt cgagggctac cggcgctttc tggcttactc
     4561 ccaggcggta ttccaggagg gatatttgcg cctcggcagc gtgccgttcc tctcttttcg
     4621 cgacatgctg cgcgccgggc cgcagctgct taagctccag gcgtggcaga gcgtctacca
     4681 gtcggtttcg cgctttattg aggatgagca tctgcggcag gccttctcgt tccactccct
     4741 gctggtaggc ggcaacccct tcaccacctc gtccatctac accctgatcc acgcccttga
     4801 gcgggagtgg ggggtctggt tccctgaggg cggcaccggg gcgctggtga acggcatggt
     4861 gaagctgttt accgatctgg gcggggagat cgaactcaac gcccgggtcg aagagctggt
     4921 ggtggccgat aaccgcgtaa gccaggtccg gctggcggat ggtcggatct ttgacaccga
     4981 cgccgtagcc tcgaacgctg acgtggtgaa cacctataaa aagctgctcg gccaccatcc
     5041 ggtggggcag aagcgggcgg cagcgctgga gcgcaagagc atgagcaact cgctgtttgt
     5101 gctctacttc ggcctgaacc agcctcattc ccagctggcg caccatacca tctgttttgg
     5161 tccccgctac cgggagctga tcgacgagat ctttaccggc agcgcgctgg cggatgactt
     5221 ctcgctctac ctgcactcgc cctgcgtgac cgatccctcg ctcgcgcctc ccggctgcgc
     5281 cagcttctac gtgctggccc cggtgccgca tcttggcaac gcgccgctgg actgggcgca
     5341 ggaggggccg aagctgcgcg accgcatctt tgactacctt gaagagcgct atatgcccgg
     5401 cctgcgtagc cagctggtga cccagcggat ctttaccccg gcagacttcc acgacacgct
     5461 ggatgcgcat ctgggatcgg ccttctccat cgagccgctg ctgacccaaa gcgcctggtt
     5521 ccgcccgcac aaccgcgaca gcgacattgc caacctctac ctggtgggcg caggtactca
     5581 ccctggggcg ggcattcctg gcgtagtggc ctcggcgaaa gccaccgcca gcctgatgat
     5641 tgaggatctg caatgagcca accgccgctg cttgaccacg ccacgcagac catggccaac
     5701 ggctcgaaaa gttttgccac cgctgcgaag ctgttcgacc cggccacccg ccgtagcgtg
     5761 ctgatgctct acacctggtg ccgccactgc gatgacgtca ttgacgacca gacccacggc
     5821 ttcgccagcg aggccgcggc ggaggaggag gccacccagc gcctggcccg gctgcgcacg
     5881 ctgaccctgg cggcgtttga aggggccgag atgcaggatc cggccttcgc tgcctttcag
     5941 gaggtggcgc tgacccacgg tattacgccc cgcatggcgc tcgatcacct cgacggcttt
     6001 gcgatggacg tggctcagac ccgctatgtc acctttgagg atacgctgcg ctactgctat
     6061 cacgtggcgg gcgtggtggg tctgatgatg gccagggtga tgggcgtgcg ggatgagcgg
     6121 gtgctggatc gcgcctgcga tctggggctg gccttccagc tgacgaatat cgcccgggat
     6181 attattgacg atgcggctat tgaccgctgc tatctgcccg ccgagtggct gcaggatgcc
     6241 gggctgaccc cggagaacta tgccgcgcgg gagaatcggg ccgcgctggc gcgggtggcg
     6301 gagcggctta ttgatgccgc agagccgtac tacatctcct cccaggccgg gctacacgat
     6361 ctgccgccgc gctgcgcctg ggcgatcgcc accgcccgca gcgtctaccg ggagatcggt
     6421 attaaggtaa aagcggcggg aggcagcgcc tgggatcgcc gccagcacac cagcaaaggt
     6481 gaaaaaattg ccatgctgat ggcggcaccg gggcaggtta ttcgggcgaa gacgacgagg
     6541 gtgacgccgc gtccggccgg tctttggcag cgtcccgttt aggcgggcgg ccatgacgtt
     6601 cacgcaggat cgcctgtagg tcggcaggct tgcgggcgta aataaaaccg aaggagacgc
     6661 agccctcccg gccgcgcacc gcgtggtgca ggcggtgggc gacgtagagc cgcttcaggt
     6721 agccccggcg cgggatcccg cctcgccgtg ctgtccggtc tcaacctgat ccgccagaat
     6781 cgagccaacg ggatcggcca gcacgaattc ggtatgcgga gagtgtttgg caaaccatgc
     6841 ctgcaagcca cccagcgtgc cgccggagcc aacgcctact accacggcat caaccctgcc
     6901 tgccagctgg tcgaacagct ccggtgcggt ggtggtggcg tgcgccagcg ggttggccgg
     6961 gttagagaac tgatcaatat agtaagcacc cggcgtctcc tctgccaggc ggcgggcgta
     7021 gtcctgatag tactccgggt ggccctttgt cacgtcggag cgggtcaggc gcacatcaac
     7081 acccagcgca cgcaggtggt agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     7141 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     7201 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     7261 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     7321 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     7381 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     7441 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     7501 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     7561 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     7621 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     7681 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     7741 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     7801 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac
     7861 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     7921 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     7981 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     8041 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     8101 cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc
     8161 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
     8221 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
     8281 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     8341 gtttcgtaaa gtctggaaac gcggaagtcc cctacgtgct gctgaagttg cccgcaacag
     8401 agagtggaac caaccggtga taccacgata ctatgactga gagtcaacgc catgagcggc
     8461 ctcatttctt attctgagtt acaacagtcc gcaccgctgt ccggtagctc cttccggtgg
     8521 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     8581 ggacaggtgc cggcagcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     8641 ccgaaacaag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
     8701 ccctgtggaa cacctacatc tgtattaacg aagcgctaac cgtttttatc aggctctggg
     8761 aggcagaata aatgatcata tcgtcaatta ttacctccac ggggagagcc tgagcaaact
     8821 ggcctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa
     8881 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga
     8941 atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca
     9001 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
     9061 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga
     9121 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     9181 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     9241 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     9301 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     9361 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     9421 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacg
//
