LOCUS       sequence-83100-e         839 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Receiving vector for PUM-HD assembly and expression in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pCMV-TTP(C147R)-GG-PUF
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..61
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
       61 gatggatctg actgccatct acgagagcct cctgtcgctg agccctgacg tgcccgtgcc
      121 atccgaccat ggagggactg agtccagccc aggctggggc tcctcgggac cctggagcct
      181 gagcccctcc gactccagcc cgtctggggt cacctcccgc ctgcctggcc gctccaccag
      241 cctagtggag ggccgcagct gtggctgggt gcccccaccc cctggcttcg caccgctggc
      301 tccccgcctg ggccctgagc tgtcaccctc acccacttcg cccactgcaa cctccaccac
      361 cccctcgcgc tacaagactg agctatgtcg gaccttctca gagagtgggc gctgccgcta
      421 cggggccaag tgccagtttg cccatggcct gggcgagctg cgccaggcca atcgccaccc
      481 caaatacaag acggaactcc gtcacaagtt ctacctccag ggccgctgcc cctacggctc
      541 tcgctgccac ttcatccaca accctagcga agacctggcg gccccgggcc accctcctgt
      601 gcttcgccag agcatcagct tctccggcct gccctctggc cgccggacct caccaccacc
      661 accaggcctg gccggccctt ccctgtcctc cagctccttc tcgccctcca gctccccacc
      721 accacctggg gaccttccac tgtcaccctc tgccttctct gctgcccctg gcacccccct
      781 ggctcgaaga gatcccaccc cagtctgttg cccctcctgc cgaagggcca ctcctatca
//
