LOCUS       sequence-83111-e         913 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Encodes module 1 with amino acids 12S-16E for recognition of nt 8G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-1SE
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(138..154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(138..154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(151..173)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(186..192,193..210,211..216))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    231..252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(369..386)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(620..639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggacatata atggaatttt cccaagacca gcatgggtcc agattcattg agctgaaact
       61 ggagcgtgcc acaccagctg agcgccagct tgtcttcaat gaaatcctcc agagagacca
      121 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      181 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      241 taactcacat taattgcatt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      301 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      601 tgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      901 aacaggatta gca
//
