LOCUS       sequence-83191-e       10322 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses full-length Multimerin-1 precursor ectodomain in mammalian
            cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His 
            tag. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: MMRN1-bio-His
FEATURES             Location/Qualifiers
     source          1..10322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             644..661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    713..768
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(767..786)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3004..3022)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3090..3194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3195..3263,3264..4055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3413..3432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4226..4814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4715..4734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4968..4985
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5102..5123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5138..5143,5144..5161,5162..5168)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5176..5192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5181..5203
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5200..5216
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5200..5216
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5487..5866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5867..6070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6021..6041
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6067..6091
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6572..6591
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      6625..6634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9926..9935
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggca gcctgcacca catcctggac gcccagaaga tgctgtggaa
      601 ccacagggac aggaacctgc ctcctctggc ccctctgggc ccccaccacc atcaccacca
      661 ttgagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg ctaataaagg
      721 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
      781 tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc
      841 cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg
      901 catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac
      961 acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga
     1021 tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga
     1081 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
     1141 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
     1201 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
     1261 aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta
     1321 tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat
     1381 tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg
     1441 ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca
     1501 gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt
     1561 ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt
     1621 gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg
     1681 gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag
     1741 tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc
     1801 cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag
     1861 tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc
     1921 atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact
     1981 ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat
     2041 aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc
     2101 cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg
     2161 taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg
     2221 ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga
     2281 ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc
     2341 acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat
     2401 atctggatag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     2461 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     2521 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     2581 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag
     2641 attagggtag tatatgctat cctaatttat atctgggtag catatactac ccaaatatct
     2701 ggatagcata tgctatccta atctatatct gggtagcata tgctatccta atctatatct
     2761 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     2821 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     2881 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     2941 gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa
     3001 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3061 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3121 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3181 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3241 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3301 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3361 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3421 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3481 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3541 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3601 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3661 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3721 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     3781 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3841 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3901 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3961 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4021 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4081 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4141 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4201 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4261 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4321 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     4381 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4441 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4501 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4561 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4621 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4681 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4741 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4801 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4861 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4921 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4981 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5041 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     5101 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     5161 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     5221 attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct tatcatcgca
     5281 gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta
     5341 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     5401 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     5461 ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     5521 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     5581 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     5641 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     5701 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     5761 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     5821 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     5881 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     5941 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6001 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6061 aagcagagct cgtttagtga accgtcagat cctcactctc ttccgcatcg ctgtctgcga
     6121 gggccagctg ttgggctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg
     6181 gatcggaaac ccgtcggcct ccgaacggta ctccgccacc gagggacctg agcgagtccg
     6241 catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta
     6301 ggctgagcac cgtggcgggc ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc
     6361 tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgag gtgaggtgtg
     6421 gcaggcttga gatccagctg ttggggtgag tactccctct caaaagcggg cattacttct
     6481 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgatctg
     6541 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     6601 caggtccaag tttaaactgc ggccgccacc atggtcggag ccagactgtt cgtgctgctg
     6661 tccagcctgt ggagcggcgg catcggcctg aacaacagca agcacagctg gaccatcccc
     6721 gaggacggca acagccagaa aaccatgccc agcgccagcg tgccccccaa caagatccag
     6781 agcctgcaga tcctgcccac caccagagtg atgagcgccg agatcgccac cacccctgag
     6841 gccagaacca gcgaggacag cctgctgaag tccaccctgc cccctagcga aacaagcgcc
     6901 cctgccgagg gcgtgagaaa ccagaccctg accagcacag agaaggccga gggggtggtc
     6961 aagctgcaga acctgaccct gcctaccaac gccagcatca agttcaaccc tggcgccgag
     7021 agcgtggtgc tgtccaacag caccctgaag tttctgcaga gcttcgcccg gaagtccaac
     7081 gagcaggcca cctccctgaa tacagtgggc ggcacaggcg gaattggcgg cgtgggagga
     7141 acaggcggag tgggcaacag agcccccaga gagacatacc tgagcagagg cgacagctca
     7201 tctagccagc ggaccgacta ccagaagtcc aacttcgaga caacccgggg caagaattgg
     7261 tgcgcctacg tgcacaccag actgagcccc accgtgatcc tggacaacca ggtcacctac
     7321 gtgcctggcg gcaaaggacc atgtggctgg acaggcggat catgtcctca gcggagccag
     7381 aaaatcagca accccgtgta ccggatgcag cacaagatcg tgaccagcct ggactggcgg
     7441 tgctgtcctg gctacagcgg ccccaagtgc cagctgagag cccaggaaca gcagagcctg
     7501 atccacacca accaggccga gagccacact gctgtcggaa gaggcgtggc agaacagcag
     7561 cagcagcagg gctgtggcga ccccgaagtg atgcagaaaa tgaccgacca ggtcaactac
     7621 caggccatga agctgaccct gctgcagaag aagatcgaca acatcagcct gaccgtgaac
     7681 gacgtgcgga acacctacag cagcctggaa ggcaaggtgt ccgaggacaa gagccgggag
     7741 ttccagtccc tgctgaaggg cctgaagtcc aagtccatca acgtgctgat ccgggacatc
     7801 gtgcgggagc agttcaagat cttccagaac gacatgcagg aaaccgtcgc ccagctgttc
     7861 aagaccgtgt cctccctgag cgaggacctg gaaagcaccc ggcagatcat ccagaaagtg
     7921 aacgagtccg tggtgtctat cgccgcacag cagaaatttg tcctcgtcca ggaaaaccgg
     7981 cccaccctga ccgacatcgt ggagctgcgg aaccacatcg tgaacgtgcg gcaggaaatg
     8041 accctgactt gcgagaagcc catcaaagaa ctggaagtga agcagaccca cctggaaggc
     8101 gccctggaac aggaacacag ccggtccatc ctgtactacg agagcctgaa caagaccctg
     8161 agcaagctga aagaggtgca cgagcagctg ctgtctaccg aacaggtgtc cgaccagaag
     8221 aatgcccctg ccgccgagtc cgtgagcaac aacgtgaccg agtacatgag caccctgcac
     8281 gagaacatca agaaacagtc cctgatgatg ctgcagatgt tcgaggatct gcacatccag
     8341 gaaagcaaga tcaacaacct gaccgtgtcc ctggaaatgg aaaaagagtc cctgcggggc
     8401 gagtgcgagg acatgctgtc caagtgccgg aacgacttca agttccagct gaaggacacc
     8461 gaagagaatc tgcacgtgct gaatcagacc ctggccgagg tgctgttccc catggacaac
     8521 aagatggaca agatgagcga gcagctgaac gacctgacct acgacatgga aatcctgcag
     8581 cccctgctgg aacagggcgc cagcctgcgg cagaccatga catacgagca gcccaaagag
     8641 gccatcgtca tccggaagaa gattgagaat ctgaccagcg ccgtgaacag cctgaacttc
     8701 atcatcaaag agctgaccaa gcggcacaat ctgctgcgga acgaggtgca gggcagggac
     8761 gacgccctgg aaagacggat caacgagtac gccctcgaga tggaagatgg cctgaataag
     8821 accatgacca tcatcaacaa cgccatcgac ttcatccagg acaactacgc cctgaaagag
     8881 acactgagca ccatcaagga caacagcgag atccaccaca agtgcaccag cgatatggaa
     8941 accatcctga ccttcatccc ccagttccac cggctgaacg acagcatcca gaccctggtc
     9001 aacgacaacc agcggtacaa cttcgtgctc caggtggcca agacactggc cggcatcccc
     9061 cgggacgaga agctgaacca gagcaacttc cagaaaatgt accagatgtt caacgagaca
     9121 accagccagg tccggaagta ccagcagaac atgtctcacc tggaagagaa gctgctgctg
     9181 accaccaaga tcagcaagaa cttcgaaacc cggctgcagg acatcgagag caaagtgacc
     9241 cagacactga tcccttacta catcagcgtg aagaaaggca gcgtggtcac caacgagagg
     9301 gaccaggccc tgcagctcca ggtgctgaac agccggttca aggccctgga agccaagagc
     9361 atccacctga gcatcaactt tttctccctg aacaaaactc tccacgaggt gctcaccatg
     9421 tgccacaacg cctccacctc cgtgagcgag ctgaacgcca ccatccccaa gtggatcaag
     9481 cacagcctgc ccgacatcca gctgctccag aaggggctga ccgagtttgt ggagcccatc
     9541 atccagatca agacccaggc cgccctgtcc aacctgacct gttgcatcga cagatctctg
     9601 cctggcagcc tggccaatgt ggtcaagagc cagaaacagg tcaagtccct gcccaagaag
     9661 atcaacgccc tgaagaaacc cacagtgaac ctgaccaccg tgctgatcgg ccggacccag
     9721 cggaacaccg acaatatcat ctaccccgag gaatacagct cctgcagccg gcacccttgt
     9781 cagaacggcg gcacctgtat caacggccgg accagcttca cctgtgcctg cagacacccc
     9841 ttcaccggcg acaactgtac catcaaactg gtggaagaga acgccctggc ccccgacttt
     9901 agcaagggca gctacagata cgcccccatg gtggcctttt tcgccagcca cacctacggc
     9961 atgaccatcc caggaccaat cctgttcaac aatctggacg tgaactacgg cgccagctac
    10021 accccccgga ccggcaagtt cagaatcccc tacctgggcg tgtacgtgtt caagtacacc
    10081 atcgagagct tcagcgccca catcagcggc tttctggtgg tggacggcat cgacaagctg
    10141 gccttcgaga gcgagaatat caactccgag atccactgcg acagagtgct gacaggcgac
    10201 gccctgctcg agctgaatta cggccaggaa gtgtggctga gactggccaa gggcaccatc
    10261 cctgccaagt tcccccccgt gaccaccttc agcggctatc tgctgtaccg gactggcgcg
    10321 cc
//
