LOCUS       sequence-83282-e       11304 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea 
            agglomerans) Eho10, and an lcyB cDNA of Arabidopsis thaliana. Use 
            with pY2F to produce alpha-carotene in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Cunningham FX Jr, Lee H, Gantt E
  TITLE     Carotenoid biosynthesis in the primitive red alga Cyanidioschyzon 
            merolae.
  JOURNAL   Eukaryot Cell. 2007 Mar;6(3):533-45. Epub 2006 Nov 3.
  PUBMED    17085635
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell."; date: "2007-03"; volume: "6(3)"; pages: "533-45. Epub 2006 
            Nov 3"
COMMENT     Sequence Label: pAC-BETA-At
FEATURES             Location/Qualifiers
     source          1..11304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1535..1551
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1535..1551
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8992..9011)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(10864..11304,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagctgg ccgctctaga actagtggat cccccgggct
     1561 gcaggaattc gatggatact ctgttgaaaa cacccaacaa gctcgatttt ttcatccctc
     1621 agtttcatgg gtttgagaga ttatgcagta acaatccata ccattcaagg gttaggcttg
     1681 gtgtgaagaa aagggctatc aaaattgtct ctagtgtagt gagtggtagc gctgctcttt
     1741 tggatcttgt tcctgaaact aagaaggaga atcttgactt tgagcttcct ttgtacgaca
     1801 cttccaagag tcaagttgtt gatttggcta ttgttggtgg tggtcctgct ggtttagccg
     1861 tggctcagca ggtttctgaa gctggactct ctgtttgttc cattgatcct tctcctaagc
     1921 tcatatggcc taacaattat ggagtttggg ttgatgagtt tgaggctatg gatttactag
     1981 actgcctgga taccacatgg tctggtgctg ttgtctatgt cgatgaaggt gtcaagaagg
     2041 atttgagccg gccttatggg agagttaacc ggaaacagct caaatccaaa atgcttcaga
     2101 aatgtattac caacggtgtt aaatttcatc agtctaaggt cactaatgtg gttcacgagg
     2161 aggcaaactc cactgtggtc tgcagtgacg gtgtaaagat tcaggcttcc gtggttcttg
     2221 atgccactgg gttttcccga tgcttggttc agtatgacaa accttacaac cctgggtacc
     2281 aagtagctta cgggattgta gctgaagttg atggtcaccc attcgatgta gacaaaatgg
     2341 tgttcatgga ttggagagac aaacatctgg actcatatcc tgagctgaaa gaacggaaca
     2401 gcaagatccc aacgttcttg tacgctatgc cattttcttc caaccgaata tttcttgaag
     2461 aaacttcttt agttgctaga cctggtctga gaatggaaga tatccaagaa agaatggctg
     2521 ctagactgaa acatctgggg atcaatgtga agaggattga ggaagacgag cgttgtgtga
     2581 tcccgatggg cggtccttta ccagtcttac ctcaacgggt tgtggggatt ggtgggacag
     2641 caggaatggt tcatccttca actggttaca tggttgctag gactcttgca gctgcaccaa
     2701 tagttgcaaa tgccattgtg agatacctcg gttcaccaag tagtaatagc ctgagaggag
     2761 atcaactctc tgctgaggtt tggagagact tgtggcctat cgaacggcgt agacagaggg
     2821 agttcttctg ttttggaatg gatattctgc tgaaactcga tttagacgct actagaaggt
     2881 tctttgatgc attctttgat ctgcaacctc attactggca cggattcttg tcttccaggc
     2941 tgtttctccc ggaactgttg gtcttcgggt tgtcgctctt ctcacacgct tccaatacct
     3001 caagattgga gatcatgaca aaggggactg ttcctcttgc taagatgatc aacaatttgg
     3061 tacaagatag agactaagga ccagaaactt agacatataa gtacatctgt tctttggttc
     3121 ttgaccagta gtatatccgc attgcaagtc gttggataat tgtgtataaa ccacagatca
     3181 taacctgaat ccttgtgaaa tcaaattgtt actactagtt cattaaaatt aatactttgt
     3241 gctgcattgt gtttcaccaa ctcttgtaaa tccaaaacta gaggcaaaat gtaataagat
     3301 aaaagagtgg attttgaaca ccaaaaaaaa aaaaaaaaaa aaaagggcgg ccagctttaa
     3361 tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac
     3421 aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt
     3481 atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat cgccagtcac
     3541 tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga
     3601 gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact
     3661 atcgactacg cgatcatggc gaccacaccc gtcctgtgga tctaaaggca cagcgtctca
     3721 tgcttcgcac aatgtaaaac tgcttcagaa cctggcgaga gctatccgcg cggtctacgg
     3781 ttaactgata ctaaaagaca attcagcggg taaccttgca atggtgagtg gcagtaaagc
     3841 gggcgtttcg cctcatcgcg aaatagaagt aatgagacaa tccattgacg atcacctggc
     3901 tggcctgtta cctgaaaccg acagccagga tatcgtcagc cttgcgatgc gtgaaggcgt
     3961 catggcaccc ggtaaacgga tccgtccgct gctgatgctg ctggccgccc gcgacctccg
     4021 ctaccagggc agtatgccta cgctgctcga tctcgcctgc gccgttgaac tgacccatac
     4081 cgcgtcgctg atgctcgacg acatgccctg catggacaac gccgagctgc gccgcggtca
     4141 gcccactacc cacaaaaaat ttggtgagag cgtggcgatc cttgcctccg ttgggctgct
     4201 ctctaaagcc tttggtctga tcgccgccac cggcgatctg ccgggggaga ggcgtgccca
     4261 ggcggtcaac gagctctcta ccgccgtggg cgtgcagggc ctggtactgg ggcagtttcg
     4321 cgatcttaac gatgccgccc tcgaccgtac ccctgacgct atcctcagca ccaaccacct
     4381 caagaccggc attctgttca gcgcgatgct gcagatcgtc gccattgctt ccgcctcgtc
     4441 gccgagcacg cgagagacgc tgcacgcctt cgccctcgac ttcggccagg cgtttcaact
     4501 gctggacgat ctgcgtgacg atcacccgga aaccggtaaa gatcgcaata aggacgcggg
     4561 aaaatcgacg ctggtcaacc ggctgggcgc agacgcggcc cggcaaaagc tgcgcgagca
     4621 tattgattcc gccgacaaac acctcacttt tgcctgtccg cagggcggcg ccatccgaca
     4681 gtttatgcat ctgtggtttg gccatcacct tgccgactgg tcaccggtca tgaaaatcgc
     4741 ctgataccgc ccttttgggt tcaagcagta cataacgatg gaaccacatt acaggagtag
     4801 tgatgaatga aggacgagcg ccttgttcag cgtaagaacg atcatctgga tatcgttctc
     4861 gacccccgtc gcgccgtaac tcaggctagc gcaggttttg agcgctggcg ctttacccac
     4921 tgcgccctgc cagagctgaa ttttagcgac atcacgctgg aaaccacctt cctgaatcgg
     4981 cagctacagg ctccgctgct gatcagctcc atgaccggcg gcgttgagcg ctcgcgccat
     5041 atcaaccgcc acctcgccga ggcggcgcag gtgctaaaaa ttgcgatggg ggtgggctcc
     5101 cagcgcgtcg ccattgagag cgacgcgggc ttagggctgg ataaaaccct gcggcagctg
     5161 gctccggacg tgccgctgct ggcgaacctc ggcgcggcgc agctgaccgg cagaaaaggt
     5221 attgattacg cccgacgggc cgtggagatg atcgaggcgg atgcgctgat tgtgcaccta
     5281 aacccgctgc aggaggcgct acagcccggc ggcgatcgcg actggcgcgg acggctggcg
     5341 gctattgaaa ctctggtccg cgagctgccc gttccgctgg tggtgaaaga ggtgggagcc
     5401 ggtatctccc gaaccgtggc cgggcagctg atcgatgccg gcgttaccgt gattgacgtc
     5461 gcgggcgcgg gcggcaccag ctgggccgcc gttgaaggcg agcgggcggc caccgagcag
     5521 cagcgcagcg tggccaacgt ctttgccgac tgggggatcc ccaccgctga ggcgctggtt
     5581 gacattgccg aggcctggcc gcagatgccc cttattgcct cgggaatgcg ggctgggcta
     5641 tttcacccta ccactggcta ttcgctgccg ctggcggtgg cccttgccga cgcgattgcc
     5701 gacagcccgc ggctgggcag cgttccgctc tatcagctca cccggcagtt tgccgaacgc
     5761 cactggcgca ggcagggatt cttccgcctg ctgaaccgga tgcttttcct ggccgggcgc
     5821 gaggagaacc gctggcgggt gatgcagcgc ttttatgggc tgccggagcc caccgtagag
     5881 cgcttttacg ccggtcggct ctctctcttt gataaggccc gcattttgac gggcaagcca
     5941 ccggttccgc tgggcgaagc ctggcgggcg gcgctgaacc attttcctga cagacgagat
     6001 aaaggatgaa aaaaaccgtt gtgattggcg caggctttgg tggcctggcg ctggcgattc
     6061 gcctgcaggc ggcagggatc ccaaccgtac tgctggagca gcgggacaag cccggcggtc
     6121 gggcctacgt ctggcatgac cagggcttta cctttgacgc cgggccgacg gtgatcaccg
     6181 atcctaccgc gcttgaggcg ctgttcaccc tggccggcag gcgcatggag gattacgtca
     6241 ggctgctgcc ggtaaaaccc ttctaccgac tctgctggga gtccgggaag accctcgact
     6301 atgctaacga cagcgccgag cttgaggcgc agattaccca gttcaacccc cgcgacgtcg
     6361 agggctaccg gcgctttctg gcttactccc aggcggtatt ccaggaggga tatttgcgcc
     6421 tcggcagcgt gccgttcctc tcttttcgcg acatgctgcg cgccgggccg cagctgctta
     6481 agctccaggc gtggcagagc gtctaccagt cggtttcgcg ctttattgag gatgagcatc
     6541 tgcggcaggc cttctcgttc cactccctgc tggtaggcgg caaccccttc accacctcgt
     6601 ccatctacac cctgatccac gcccttgagc gggagtgggg ggtctggttc cctgagggcg
     6661 gcaccggggc gctggtgaac ggcatggtga agctgtttac cgatctgggc ggggagatcg
     6721 aactcaacgc ccgggtcgaa gagctggtgg tggccgataa ccgcgtaagc caggtccggc
     6781 tggcggatgg tcggatcttt gacaccgacg ccgtagcctc gaacgctgac gtggtgaaca
     6841 cctataaaaa gctgctcggc caccatccgg tggggcagaa gcgggcggca gcgctggagc
     6901 gcaagagcat gagcaactcg ctgtttgtgc tctacttcgg cctgaaccag cctcattccc
     6961 agctggcgca ccataccatc tgttttggtc cccgctaccg ggagctgatc gacgagatct
     7021 ttaccggcag cgcgctggcg gatgacttct cgctctacct gcactcgccc tgcgtgaccg
     7081 atccctcgct cgcgcctccc ggctgcgcca gcttctacgt gctggccccg gtgccgcatc
     7141 ttggcaacgc gccgctggac tgggcgcagg aggggccgaa gctgcgcgac cgcatctttg
     7201 actaccttga agagcgctat atgcccggcc tgcgtagcca gctggtgacc cagcggatct
     7261 ttaccccggc agacttccac gacacgctgg atgcgcatct gggatcggcc ttctccatcg
     7321 agccgctgct gacccaaagc gcctggttcc gcccgcacaa ccgcgacagc gacattgcca
     7381 acctctacct ggtgggcgca ggtactcacc ctggggcggg cattcctggc gtagtggcct
     7441 cggcgaaagc caccgccagc ctgatgattg aggatctgca atgagccaac cgccgctgct
     7501 tgaccacgcc acgcagacca tggccaacgg ctcgaaaagt tttgccaccg ctgcgaagct
     7561 gttcgacccg gccacccgcc gtagcgtgct gatgctctac acctggtgcc gccactgcga
     7621 tgacgtcatt gacgaccaga cccacggctt cgccagcgag gccgcggcgg aggaggaggc
     7681 cacccagcgc ctggcccggc tgcgcacgct gaccctggcg gcgtttgaag gggccgagat
     7741 gcaggatccg gccttcgctg cctttcagga ggtggcgctg acccacggta ttacgccccg
     7801 catggcgctc gatcacctcg acggctttgc gatggacgtg gctcagaccc gctatgtcac
     7861 ctttgaggat acgctgcgct actgctatca cgtggcgggc gtggtgggtc tgatgatggc
     7921 cagggtgatg ggcgtgcggg atgagcgggt gctggatcgc gcctgcgatc tggggctggc
     7981 cttccagctg acgaatatcg cccgggatat tattgacgat gcggctattg accgctgcta
     8041 tctgcccgcc gagtggctgc aggatgccgg gctgaccccg gagaactatg ccgcgcggga
     8101 gaatcgggcc gcgctggcgc gggtggcgga gcggcttatt gatgccgcag agccgtacta
     8161 catctcctcc caggccgggc tacacgatct gccgccgcgc tgcgcctggg cgatcgccac
     8221 cgcccgcagc gtctaccggg agatcggtat taaggtaaaa gcggcgggag gcagcgcctg
     8281 ggatcgccgc cagcacacca gcaaaggtga aaaaattgcc atgctgatgg cggcaccggg
     8341 gcaggttatt cgggcgaaga cgacgagggt gacgccgcgt ccggccggtc tttggcagcg
     8401 tcccgtttag gcgggcggcc atgacgttca cgcaggatcg cctgtaggtc ggcaggcttg
     8461 cgggcgtaaa taaaaccgaa ggagacgcag ccctcccggc cgcgcaccgc gtggtgcagg
     8521 cggtgggcga cgtagagccg cttcaggtag ccccggcgcg ggatcccgcc tcgccgtgct
     8581 gtccggtctc aacctgatcc gccagaatcg agccaacggg atcggccagc acgaattcgg
     8641 tatgcggaga gtgtttggca aaccatgcct gcaagccacc cagcgtgccg ccggagccaa
     8701 cgcctactac cacggcatca accctgcctg ccagctggtc gaacagctcc ggtgcggtgg
     8761 tggtggcgtg cgccagcggg ttggccgggt tagagaactg atcaatatag taagcacccg
     8821 gcgtctcctc tgccaggcgg cgggcgtagt cctgatagta ctccgggtgg ccctttgtca
     8881 cgtcggagcg ggtcaggcgc acatcaacac ccagcgcacg caggtggtag atcctctacg
     8941 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     9001 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     9061 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     9121 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     9181 tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca
     9241 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     9301 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     9361 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     9421 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     9481 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     9541 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     9601 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     9661 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg
     9721 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
     9781 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
     9841 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
     9901 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag
     9961 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
    10021 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
    10081 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
    10141 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcccc
    10201 tacgtgctgc tgaagttgcc cgcaacagag agtggaacca accggtgata ccacgatact
    10261 atgactgaga gtcaacgcca tgagcggcct catttcttat tctgagttac aacagtccgc
    10321 accgctgtcc ggtagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
    10381 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgccca acagtccccc
    10441 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ccctgcacca ttatgttccg
    10501 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
    10561 gcgctaaccg tttttatcag gctctgggag gcagaataaa tgatcatatc gtcaattatt
    10621 acctccacgg ggagagcctg agcaaactgg cctcaggcat ttgagaagca cacggtcaca
    10681 ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga
    10741 ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct
    10801 tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa
    10861 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
    10921 gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt
    10981 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
    11041 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
    11101 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
    11161 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
    11221 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
    11281 ctcaccgtct ttcattgcca tacg
//
