LOCUS       sequence-83330-e         951 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             78..110
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(865..883)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(866..883)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(903..920)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttatg gctagcatga ctggtggaca gcaaatgggt ggatccgagt
      121 cctttgggct gttctccagc gtcatcaatg gggaggagca tgagcaaacc catcgggcta
      181 tattcaggtt tgtgcctcgg catgaagatg aacttgagct ggaagtggat gaccccctgc
      241 tggtggagct gcaggcagaa gactattggt atgaggccta taacatgcgc accggagccc
      301 gcggggtctt ccctgcctac tatgccattg aggtcaccaa ggagcctgag cacatggcag
      361 cccttgccaa aaacagcgac tggattgacc agttccgggt gaagttcctg gggtctgtcc
      421 aggttcctta tcacaagggc aatgatgtcc tctgtgctgc tatgcaaaag atcgccacca
      481 cccgccggct caccgtgcac tttaacccgc cctccagctg tgtccttgag atcagtgtca
      541 ggggtgtcaa gataggcgtc aaagctgatg atgctctgga ggccaaggga aataaaagta
      601 gccacttctt ccagctaaag aacatctctt tctgtggata ccatccaaag aataacaagt
      661 actttgggtt tatcactaag caccctgctg accaccggtt tgcctgccat gtctttgtgt
      721 ctgaagattc caccaaagcc ctggcggagt ctgtggggcg tgcatttcag cagttctaca
      781 agcagtttgt ggagtatacc actcctacag aagatatcta cttggagtag gcggccgctc
      841 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
      901 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc c
//
