LOCUS       sequence-83502-e         901 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Can be used to generate AAV virus that will express cytoplasmic 
            tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence 
            of Cre in neurons from the synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Oh SW, Harris JA, Ng L, Winslow B, Cain N, Mihalas S, Wang Q, Lau C,
            Kuan L, Henry AM, Mortrud MT, Ouellette B, Nguyen TN, Sorensen SA, 
            Slaughterbeck CR, Wakeman W, Li Y, Feng D, Ho A, Nicholas E, 
            Hirokawa KE, Bohn P, Joines KM, Peng H, Hawrylycz MJ, Phillips JW, 
            Hohmann JG, Wohnoutka P, Gerfen CR, Koch C, Bernard A, Dang C, Jones
            AR, Zeng H
  TITLE     A mesoscale connectome of the mouse brain.
  JOURNAL   Nature. 2014 Apr 10;508(7495):207-14. doi: 10.1038/nature13186. Epub
            2014 Apr 2.
  PUBMED    24695228
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13186"; journalName: "Nature"; date: "2014-04-10- 
            10"; volume: "508"; issue: "7495"; pages: "207-14"
COMMENT     Sequence Label: AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             791..844
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cgagcgctcc gagggccgcc accacctgtt cctggggcat ggtaccggca gcaccggcag
       61 cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtgatta aggagtttat
      121 gaggtttaaa gtcaggatgg agggcagcat gaacgggcac gagtttgaga ttgaaggaga
      181 gggggaaggg aggccctatg agggcaccca gacagcaaag ctgaaagtga caaagggcgg
      241 ccctctgcca ttcgcatggg acatcctgtc tcctcagttt atgtatggaa gtaaagccta
      301 cgtgaaacac cctgctgaca ttccagatta caagaaactg agcttcccag aggggtttaa
      361 gtgggaaaga gtgatgaact tcgaagacgg gggcctggtg actgtcaccc aggacagctc
      421 tctgcaggac ggcactctga tctataaggt gaaaatgcgc ggcaccaatt ttccccctga
      481 tggcccagtc atgcagaaga agacaatggg ctgggaggct tcaacagaaa ggctgtaccc
      541 cagagacggc gtgctgaagg gagagatcca ccaggcactg aagctgaaag atggagggca
      601 ttatctggtc gaattcaaaa ccatctacat ggccaagaaa cccgtgcagc tgcctggcta
      661 ctattacgtc gacacaaagc tggatattac tagccataac gaagactaca ccattgtgga
      721 gcagtatgag cggagcgagg ggaggcacca tctgtttctg tacggcatgg acgagctgta
      781 caagactagt gagggcagag gaagtcttct gacatgcgga gacgtggaag agaatcccgg
      841 ccctgcccca ggctcatcta gaatggacgt ggtgaatcag ctggtggctg ggggtcagtt
      901 c
//
