LOCUS       sequence-83607-e        4688 bp    DNA     circular SYN 14-APR-2025
DEFINITION  mammalian expression vector with Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Kuo WT, Lee TC, Yang HY, Chen CY, Au YC, Lu YZ, Wu LL, Wei SC, Ni 
            YH, Lin BR, Chen Y, Tsai YH, Kung JT, Sheu F, Lin LW, Yu LC
  TITLE     LPS receptor subunits have antagonistic roles in epithelial 
            apoptosis and colonic carcinogenesis.
  JOURNAL   Cell Death Differ. 2015 Jan 30. doi: 10.1038/cdd.2014.240.
  PUBMED    25633197
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2015 Jan 30. doi: 10.1038/cdd.2014.240."
COMMENT     Sequence Label: pMyc-CMV1
FEATURES             Location/Qualifiers
     source          1..4688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     128..150
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     142..159
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     143..159
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        312..690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        691..894
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     845..865
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             925..969
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             970..999
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1027..1651
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1673..1693)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1678..2008
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1859..1994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2046..2065)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2047..2065)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2079..2095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2079..2095)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2092..2114)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2103..2119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2127..2133,2134..2151,2152..2157))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2172..2193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2310..2327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2481..3069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2561..2580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3240..4031,4032..4100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3863..3882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4101..4205)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4232..4688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4319..4338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4529..4550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccca
      121 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgat ctaatcaata
      181 ttggccatta gccatattat tcattggtta tatagcataa atcaatattg gctattggcc
      241 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
      301 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      361 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      421 cgaccgccca gcgacccccg cccgttgacg tcaatagtga cgtatgttcc catagtaacg
      481 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      541 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
      601 tggcccgcct agcattatgc ccagtacatg accttacggg agtttcctac ttggcagtac
      661 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
      721 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      781 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
      841 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      901 gtgaaccgtc agaattaatt caccatgtct gcacttctga tcctagctct tgttggagct
      961 gcagttgctg aacaaaaact catctcagaa gaggatctgc ttgcggcctc gactctagag
     1021 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggcctt ggaagttgcc
     1081 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     1141 gtgtcctcta taatattatg gggtggaggg gggtggtatg gagcaagggg cccaagttgg
     1201 gaagacaacc tgtagggcct gcggggtcta ttcgggaacc aagctggagt gcagtggcac
     1261 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
     1321 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
     1381 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
     1441 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
     1501 tccttctgat tttaaaataa ctataccagc aggaggacgt ccagacacag cataggctac
     1561 ctgccatggc ccaaccggtg ggacatttga gttgcttgct tggcactgtc ctctcatgcg
     1621 ttgggtccac tcagtagatg cctgttgaat tgggtacgcg gccagcttct gtggaatgtg
     1681 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     1741 catctcaatt agtcagcaac caggtgtgga aaagtcccca ggctccccag caggcagaag
     1801 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     1861 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     1921 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     1981 cttttttgga ggcctaggct tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt
     2041 taattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt tcctgtgtga
     2101 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2161 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2221 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2281 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2641 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     2701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2881 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3121 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3181 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4201 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     4261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4321 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4381 aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4441 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4501 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4561 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4621 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaaacgtt
     4681 tacaattt
//
